No abstract

En este trabajo se evaluaron los ensayos para la obtención de azúcares fermentables a partir de materias amiláceas, en este caso particular yuca, los cuales comprendieron el seguimiento de la reacción de hidrólisis enzimática para la posterior determinación de la cinética de reacción (formación de producto en función del tiempo). En una primera etapa se consideró el proceso convencional que consta de licuefacción y sacarificación, a cargo de una alfa-amilasa y una gluco-amilasa comerciales respectivamente. Yen una segunda etapa la acción bifuncional de una única enzima comercial, analizándose por separado las dos partes constitutivas de la yuca: el parénquima interno y el parénquima externo. La materia prima se caracterizó en cuanto a su composición, porcentaje de humedad, proteína y por último contenido de almidón enbase seca.

This work presents the simulation in Aspen Plusr of a process to obtain arabinoxylans (AX) from Brewer’s Spent Grain (BSG), which is the major byproduct of the brewing industry. The process is divided into two stages: alkaline pretreatment and enzymatic hydrolysis. These stages cover the extraction of proteins and AX from BSG using an alkaline pretreatment and enzymatic hydrolysis of the AX separated from the liquid stream to obtain xylose, i.e. the substrate required for the fermentation to xylitol. Simulation results show that xylose obtained corresponds to 8,5% of the dry weight of the raw material, obtaining a yield of 58%. Several streams of byproducts were obtained, such as proteins, polypeptides, amino acids, phenolic compounds and lignocellulosic residues that can be valorized in other processes. Simulation was performed in the context of a biorefinery in Colombia.

Xylose is an abundant bioresource for obtaining diverse chemicals and added-value products. The production of xylose from green alternatives like enzymatic hydrolysis is an important step in a biorefinery context. This research evaluated the synergism among four classes of hydrolytic purified enzymes—endo-1,4-β-xylanase, α-l-arabinofuranosidase, β-xylosidase, and α-d-glucuronidase—over hydrolysis of glucuronoarabinoxylan (GAX) obtained from brewers’ spent grain (BSG) after alkaline extraction and ethanol precipitation. First, monosaccharides, uronic acids and glycosidic-linkages of alkaline extracted GAX fraction from BSG were characterized, after that different strategies based on the addition of one or two families of enzymes—endo-1,4-β-xylanase (GH10 and GH11) and α-l-arabinofuranosidase (GH43 and GH51)—cooperating with one β-xylosidase (GH43) and one α-d-glucuronidase (GH67) into enzymatic hydrolysis were assessed to obtain the best yield of xylose. The xylose release was monitored over time in the first 90 min and after a prolonged reaction up to 48 h of reaction. The highest yield of xylose was 63.6% (48 h, 40 ℃, pH 5.5), using a mixture of all enzymes devoid of α-l-arabinofuranosidase (GH43) family. These results highlight the importance of GH51 arabinofuranosidase debranching enzyme to allow a higher cleavage of the xylan backbone of GAX from BSG and their synergy with 2 endo-1,4-β-xylanase (GH10 and GH11), one β-xylosidase (GH43) and the inclusion of one α-d-glucuronidase (GH67) in the reaction system. Therefore, this study provides an environmentally friendly process to produce xylose from BSG through utilization of enzymes as catalysts. Graphical Abstract