Spodoptera frugiperda J. E. Smith (Lepidoptera: Noctuidae) is a new pest in maize plants in Indonesia. This insect is originally from America and has spread to several countries causing yield losses. In the early 2019, this pests has attacked maize plants in the Sumatra region. This study aimed to know the presence of S. frugiperda in Bandung, Garut and Sumedang region. Surveys and observations were conducted in 14 subdistricts (6 subdistricts in Bandung, 6 subdistricts in Garut, and 2 subdistricts in Sumedang). The results showed that S. frugiperda has attacked maize plants in Bandung (Soreang), Garut (Leles, Banyuresmi, and Sucinaraja), and Sumedang (Jatinangor). Identification based on the typical morphological characters found in S. frugiperda larvae, such as the form of "Y" inverted on the head capsule and the patterns of black spots on the abdominal segments (square and trapezoidal forms). S. frugiperda larvae attacked plant growing points but the population was still low. The damage caused by S. frugiperda on maize was very similar to that caused by Mythimna separata, which was commonly found in the survey location of Bandung and Garut. It was looked different, the abundance of S. frugiperda larvae in Cilayung, Sumedang district was quite high. AbstrakKasus Serangan Ulat Grayak Jagung Spodoptera frugiperda J. E. Smith (Lepidoptera: Noctuidae) pada Tanaman Jagung di Kabupaten Bandung, Garut dan Sumedang, Jawa Barat

Conidial density and viability of Beauveria bassiana and Metarhizium anisopliae grown on the low-pH in vitro medium. Liquid bioinsecticide with active ingredient from conidial entomopathogenic fungus has major constraints, namely short shelf life due to declining conidial viability and density is caused by low pH in the bioinsecticide carrier. This experiment aimed to measure the loss of conidial viability and density of Beauveria bassiana and Metarhizium anisopliae isolates grown on in vitro medium with low pH. Entomopathogenic fungus isolates were used as much as 28 isolates grown on in vitro medium at low pH, namely pH 5, 4, 3, and 2. The results showed that the fungus isolate that had the highest conidial density on in vitro medium at pH 5 was found on isolates of B. bassiana with code BPcMs (2.583 x 10 9 conidia mL -1 ), while the lowest one was found on isolates of B. bassiana with code of BWS Pantura (0.825x10 9 conidia mL -1 ). All isolate conidial density from in vitro medium with pH 2 decreased regularly. Conidial density of BPcMs isolate decreased to 2.483 x 10 9 conidia mL -1, as well as BWS Pantura isolate also decreased to 0.425x10 9 conidia mL -1 . The highest conidial viability at pH 5 was found on isolates of B. bassiana with code of BPcMs (51.572%), while the lowest conidial viability was found on isolate of B. bassiana with BTmPc code (15.040%). At pH 2, almost isolates tested had low conidial viability. The conidial viability of isolates BPcMs decreased to 47.037%%, while the isolates BTmPc also decreased to 12.778%. Therefore, the lower of the pH of the in vitro medium was, the lower of conidial viability and density of B. bassiana and M. anisopliae was. Key words: isolate, bioinsecticide, shelf life ABSTRAKKerapatan dan viabilitas konidia Beauveria bassiana dan Metarhizium anisopliae pada media in vitro pH rendah. Bioinsektisida cair berbahan aktif konidia jamur entomopatogen memiliki kendala utama, yaitu singkatnya umur simpan yang diakibatkan menurunnya viabilitas dan kerapatan konidia yang salah satu penyebabnya adalah penurunan pH media pembawa. Penelitian ini bertujuan untuk mengukur penurunan viabilitas dan kerapatan konidia isolat-isolat Beauveria bassiana dan Metarhizium anisopliae yang ditumbuhkan pada media in vitro pada pH rendah. Isolat yang digunakan sebanyak 28 isolat yang ditumbuhkan pada media in vitro pada pH rendah, yaitu pH 5, 4, 3, dan 2. Hasil penelitian menunjukkan pada media in vitro pada pH 5 isolat yang memiliki kerapatan tertinggi ditemukan pada isolat B. bassiana kode BPcMs (2,583x10 9 konidia mL -1 ), sedangkan terendah pada isolat B. bassiana kode Bws Pantura (0,825x10 9 konidia mL -1 ). Semua isolat pada media in vitro pH 2 menurun teratur kerapatan konidianya. Isolat BPcMs menurun kerapatannya menjadi 2,483 x10 9 konidia mL -1 , begitu juga isolat Bws Pantura turun menjadi 0,425x10 9 konidia mL -1 . Viabilitas konidia pada pH 5 tertinggi ditemukan pada isolat B. bassiana kode BPcMs (51,572%), sedangkan terendah pada isolat B. bassiana kode BTmPc (15,04...

Lalat buah (Diptera: Tephritidae), Bactrocera cucurbitae (Coquillet) dan Bactrocera dorsalis (Hendel), merupakan hama bersifat polifag dan menjadi penyebab kerugian ekonomi berbagai buah dan sayuran di Indonesia. Tujuan dari penelitian ini adalah untuk mengetahui kisaran inang OPT di Indonesia dan pengaruh berbagai jenis buah-buahan terhadap kemampuan oviposisi dan kelangsungan hidup keturunannya. Metode penelitian menggunakan Choice test dengan menggunakan buah-buahan yang berbeda seperti apel hijau (apel malang), belimbing, pisang, jambu biji dan jeruk. Penelitian dilakukan di Laboratorium Hama Tanaman Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran. Penelitian ini menggunakan dua spesies lalat buah koleksi hasil pemeliharaan Laboratorium Hama Tanaman generasi ke-2. Hasil penelitian menunjukkan bahwa B. cucurbitae dapat berkembang baik pada buah pisang dan belimbing dengan jumlah dewasa 4,25 ± 1,70 / buah dan 7,50 ± 1,90 / buah, sedangkan B. dorsalis pada buah jambu biji dengan jumlah dewasa 14,25 ± 1,55 / buah. Dapat disimpulkan secara keseluruhan B. dorsalis memiliki kisaran inang yang lebih luas dibandingkan B. cucurbitae. Kata kunci: B. cucurbitae, B. dorsalis, preferensi, tanaman inang.

Laba-laba merupakan salah satu predator penting dalam mengatur populasi serangga hama di ekosistem padi. Aplikasi pupuk ampas bungkil mimba dan kompos gulma siam diharapkan meningkatkan keberadaan laba-laba. Tujuan penelitian adalah untuk menganalisis kelimpahan dan keanekaragaman laba-laba pada ekosistem sawah padi hitam berpupuk organik asal ampas bungkil mimba dan gulma siam. Percobaan dilaksanakan di Cinenggang, Desa Cileles, Kecamatan Jatinangor, Kabupaten Sumedang, sejak bulan Mei – November 2018. Penelitian dilakukan dengan metode Rancangan Acak Kelompok (RAK) dengan lima perlakuan yaitu Kontrol, NPK, Pupuk Ampas Mimba, Kompos Gulma Siam, dan Pupuk Kandang Sapi dengan dilakukan enam ulangan. Hasil penelitian diperoleh 8 famili laba-laba yaitu, Tetragnathidae, Pholcidae, Linyphiidae, Lycosidae, Theridiidae, Oxyopidae, Clubionidae dan Araneidae. Famili yang paling dominan pada semua perlakuan adalah Tetragnathidae, dan selanjutnya diikuti oleh Famili Pholcidae dan Linyphiidae. Jumlah total laba-laba, indeks keragaman, kelimpahan, kekayaan dan kemerataan spesies diketahui cenderung lebih tinggi pada perlakuan pupuk ampas bungkil mimba dan kompos gulma siam daripada  perlakuan NPK dan kontrol.

Chromolaena odorata (L.) is a organic material as potential compost and botanical pesticides since it compounds have the potential as a pesticides is secondary metabolite. The aim of this research was to know the effects of siam weed Chromolaena odorata compost on production of secondary metabolites in the chili. The experiment was arranged in complete randomized design consisted of four treatments (no fertilizer, cow manure, siam weed compost and inorganic fertilizers) and four replications. The research was done by comparing the chemical content of leaves analyzed by Gas Chromatography-Mass Spectrometry (GC-MS) and phytochemical test. This study was conducted from September 2010 to Februari 2011, in the green house of Departement Plant Pest & Diseases, Faculty of Agriculture. In order to the extraction and characterization of secondary metabolites was conducted at the Laboratory of Chemistry, Universitas Gadjah Mada.  Overall, the results of study showed that C. odorata compost could give positive effect on production of secondary metabolites as follow terpenoid, alkaloid, steroid and flavonoid on chili