The capsular polysaccharide (CPS) of Vibrio cholerae 0139 synonym Bengal, which is thought to carry determinants of 0-specificity, was isolated by phenol/water extraction followed by delipidation of the contaminating lipopolysaccharide at pH 4.2 and gel-permeation chromatography. The CPS contained, and phosphate. The CPS was studied by NMR spectroscopy, methylation analysis, and selective degradations, including partial acid hydrolysis at pH 3.1 and dephosphorylation with aqueous 48 % hydrofluoric acid, which both resulted in complete cleavage of Col. It was concluded that the CPS is built up of hexasaccharide repeating units containing inter alia D-galaCtOSe 4,6-cyclophosphate and having the following structureThese data basically confirm the structure of the K cholerae CPS proposed on the basis of an NMR study [L. M. Preston et al. (1995) J. Bacteriol. 177, and specify exactly the absolute configurations of the constituent monosaccharides and the position of the cyclic phosphate.

The capsular polysaccharide (CPS) of Vibrio cholerae 0139 synonym Bengal, which is thought to carry determinants of 0-specificity, was isolated by phenol/water extraction followed by delipidation of the contaminating lipopolysaccharide at pH 4.2 and gel-permeation chromatography. The CPS contained, and phosphate. The CPS was studied by NMR spectroscopy, methylation analysis, and selective degradations, including partial acid hydrolysis at pH 3.1 and dephosphorylation with aqueous 48 % hydrofluoric acid, which both resulted in complete cleavage of Col. It was concluded that the CPS is built up of hexasaccharide repeating units containing inter alia D-galaCtOSe 4,6-cyclophosphate and having the following structureThese data basically confirm the structure of the K cholerae CPS proposed on the basis of an NMR study [L. M. Preston et al. (1995) J. Bacteriol. 177, and specify exactly the absolute configurations of the constituent monosaccharides and the position of the cyclic phosphate.