Anthropogenic activities such as oil exploration have resulted in an environmental concern as they are comprised of residual hydrocarbons and metals. Following the hypothesis that endogenous bacterial communities have enhanced tolerance to heavy metals, we isolated and characterized culturable lead-resistant bacteria from an oil wastewater sample and determined whether they could reduce lead ions from the medium. The wastewater sample containing indigenous bacteria were taken out from a traditional oil field, Bojonegoro District, East Java, Indonesia, and bacteria were cultured Halomonas complex (HMC) medium containing lead (II) chloride (PbCl2) with different concentrations. Bioaccumulation of lead by heavy-metals resistant bacteria was determined by using atomic absorption spectrophotometry (AAS). Our result found 21 bacterial strains that resist lead ions, of which one strain (RPb5-3) highly resisted to 10 mM. This bacterial strain also exhibited the highest accumulation of Pb, and it could grow at various temperatures, or more than their original environment. The bacterial strains could be used for bioremediation of lead toxicity, especially in oil pollutants.

TRAINING ON MAKING NATA DE COCO FOR PKK ORGANISATION IN TASIKMADU, LOWOKWARU, MALANG CITY. One of the functional food products is nata. Nata is a collection of cellulose which has a chewy texture, white, producing gel sheets that float on the liquid surface. Material that is often used as a medium for making nata is coconut water, known as Nata de Coco. Acetobacter xylinum bacteria are added in the process of making nata de coco on steril condition. The process is important to be informed to the community, in particular the PKK organization through community development program. The methods used include lectures, practice and evaluation. Survey of partners showed that 23,8% had known the process of making nata de coco while 76,2% had not known the process, through this training, 68% partners strongly agree nata production is used as a business idea.

GUIDING OF PKK MOTHERS IN LOWOKWARU MALANG DISTRICT THROUGH TRAINING FOR MAKING VEGETABLE PESTICIDES AS A CONTROL OF HYDROPONIC VEGETABLE PEST. In general, chemical pesticides are used to eradicate pests in vegetables. That is because many chemical pesticides are sold in the market and very effective in eradicating pests. The users of chemical pesticides do not understand, if the use of chemical pesticides for a long time and continuously will be very dangerous. Steps that can be taken to prevent the dangers of chemical pesticides is using natural or vegetable pesticides. Vegetable pesticides are more environmentally friendly, the prices are much cheaper, and not dangerous, and the manufacturing process is very easy. The social role of PKK mothers is carried out to improve the activities of coaching, education, training, and counseling. Therefore, it is necessary to conduct socialization and training in the manufacture of plant-based pesticides for PKK mothers in Lowokwaru District, Malang. The methods used include counseling through lectures, direct practice of making vegetable pesticides, and evaluating the process of making vegetable pesticides. The results of a survey of partners showed that 63% of PKK present did not know about vegetable pesticides. In addition, 81% of partners before participating in training activities had never been aware of the process of making vegetable pesticides. Around 90% of partners are known to have never participated in training in making vegetable pesticides. After participating in training and practice in making vegetable pesticides, it is known that 100% of the community service partners have stated that they can increase their knowledge, skills and practice by making vegetable pesticides at home.