[1] Glycerol dialkyl glycerol tetraether (GDGT) membrane lipids in surface sediments from 24 lakes in China and Nepal were analyzed. The result shows that branched GDGT lipids occur widely in the studied lake sediments. The different distribution of branched GDGTs between the Chinese soils from Weijers et al. (2007b) and the Chinese lake sediments indicates that the branched GDGTs in the lacustrine sediment are the mixture of allochthonous and autochthonous branched GDGTs. Our result supports the suggestion that temperature is the principal factor affecting the distribution of GDGTs. Seasonality, sources, and precipitation increase the uncertainty of the correlation between the methylation index of branched tetraethers (MBT)/cyclization ratio of branched tetraethers (CBT) proxy and the temperature. The correlations of the MBT/CBT index with mean annual air temperature (T) for the global lakes are T = 6.803 − 7.062 × CBT + 37.090 × MBT (n = 139, p < 0.0001, R 2 = 0.62, and RMSE = 5.24°C); for the global lakes with pH < 8.5, T = 3.949 − 5.593 × CBT + 38.213 × MBT (n = 100, p < 0.0001, R 2 = 0.73, and RMSE = 4.27°C). The significant correlation suggests that the MBT/CBT proxy might be used as a potential paleotemperature proxy in the limnic environment. The MBT/CBT proxy provides an independent way to evaluate and verify the sensitivities of the different proxies for paleotemperature reconstruction from lacustrine sediments.

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