Objective To investigate the relationship between inflammation and autophagy in high-fat obese rats by applying three intervention methods (aerobic exercise/orlistat/food restriction). Methods Male SD rats were randomly divided into normal control group (NC group, n = 10) and high fat model group (HC group, n = 67). 40 rats (n = 58) were randomly divided into high fat control group (HC group, n = 10), high fat exercise group (HE group, n = 10), high fat orlistat group (HO group, n = 10), high fat diet group (HR group, n = 10). After 8 weeks, the weight and body length of rats were measured, and Lee's index and body fat ratio were calculated; Four blood lipids in rat serum were detected by Elisa method. Inflammatory factors IL-6 and TNF-α in testicular tissue were detected by Elisa method. The expression levels of autophagic protein AKT and mTOR mRNA in testis were detected by RT-PCR. Results (1) Compared with NC group, the body weight and Lee's index of rats in HC group increased significantly (P < 0.01), and the body fat ratio increased significantly (P < 0.05); The body weight in HR group was significantly lower than that in HE and HO groups (P < 0.01). (2) Compared with NC group, the levels of TC, TG and LDL-C in serum of HC group increased significantly(P < 0.01), while the level of HDL-C decreased significantly(P < 0.01); Compared with HC group, the levels of TC and TG in HR and HE groups decreased significantly(P < 0.01), while the level of HDL-C in HR group increased significantly(P < 0.01). (3) The level of IL-6 in testicular tissue of HC group was significantly higher than that of NC group(P < 0.01). Compared with HC group, the level of IL-6 in HE and HR groups decreased significantly(P < 0.01), and the level of TNF-α in HR group decreased significantly(P < 0.05). (4) The expression level of AKT mRNA in testicular tissue of HC group was significantly higher than that of NC group(P < 0.05). Compared with HC, the expression level of each index in HO and HR groups was significantly increased(P < 0.01), and the AKT mRNA level in HE group was significantly higher than that in HC group(P < 0.05). Conclusions (1) The obese rat model reproduced in this study is successful. The three intervention methods can inhibit the weight growth of rats and effectively improve the dyslipidemia, of which the effect of food restriction intervention is the most significant. (2) The three intervention methods can effectively alleviate the inflammatory level in the testis of obese rats; The effect of aerobic exercise and food restriction intervention on reducing the level of inflammation is better than that of orlistat drug intervention. (3) Compared with food restriction intervention, exercise intervention has a greater impact on AKT and mTOR mRNA expression levels. The level of inflammatory factors is negatively correlated with the expression of autophagy related proteins, and autophagy level has a high degree of tissue specificity.