Escherichia coli Stbl4 is widely used as a laboratory strain for heterologous expression of large gene clusters. Since no genome sequence has been publicly available, we here report the draft sequence of Stbl4, including its F-plasmid. It should serve as a useful reference for researchers working with Stbl4.

Introduction:The prevalence of type 2 diabetes mellitus (T2DM) in young adults has increased considerably in the past decade. Evidence shows that lifestyle modification programs such as the Diabetes Prevention Program (DPP) are effective in improving health behaviors and lowering the risk of diabetes. DPP follow-up studies have found that a lack of retention can reduce the effectiveness of such programs. Adding a positive social reinforcement platform is a novel strategy to provide educational resources to facilitate lifestyle changes. However, evidence regarding the effect of positive social reinforcement as a theory-based strategy to prolong retention within the DPP is limited. The current study protocol aims to evaluate the efficacy of the online Prevent Diabetes® Program, including a Positive Inter@ctiOn forum on time-to-attrition from the DPP among college students at high risk for T2DM. Methods: This study protocol is a phase 3, multicenter, superiority, controlled trial conducted in West Virginia, Alabama, and Mississippi. Four hundred eigclthty college students will be centrally randomized in a cluster-wise 1:1 ratio to the online Prevent Diabetes® Program integrated with the Positive Inter@ctiOn forum or to the online Prevent Diabetes® Program alone for 12 months. The primary outcome is time-to-attrition from the DPP. Discussion: Successful completion of the study will provide valuable information on whether implementing theory-based health behavior strategies can improve the retention of participants in the online DPP and contribute to enhancing DPP effectiveness.