The ketone bodies (KBs) D‐3‐hydroxybutyrate (D‐3HB) and acetoacetate (AcAc) play a role in starvation and have been associated with insulin resistance. The dose–response relationship between insulin and KBs was demonstrated to be shifted to the right in type 2 diabetes patients. However, KB levels have also been reported to be decreased in obesity. We investigated the metabolic adaptation to fasting with respect to glucose and KB metabolism in lean and obese men without type 2 diabetes using stable glucose and D‐3HB isotopes in a two‐step pancreatic clamp after 38 h of fasting. We found that D‐3HB fluxes in the basal state were higher in lean compared to obese men: 15.2 (10.7–27.1) vs. 7.0 (3.5–15.1) µmol/kg lean body mass (LBM)·min, respectively, P < 0.01. No differences were found in KB fluxes between lean and obese volunteers during the pancreatic clamp (step 1: 6.9 (1.8–12.0) vs. 7.4 (4.2–17.8) µmol/kg LBM·min, respectively; and step 2: 2.9 (0–7.2) vs. 3.4 (0.85–18.7) µmol/kg LBM·min, respectively), despite similar plasma insulin levels. Meanwhile, peripheral glucose uptake was higher in lean compared to obese men (step 1: 15.2 (12.3–25.6) vs. 14.7 (11.9–22.7) µmol/kg LBM·min, respectively, P ≤ 0.05; and step 2: 12.5 (7.0–17.3) vs. 10.8 (5.2–15.0) µmol/kg LBM·min, respectively, P ≤ 0.01). These data show that obese subjects who display insulin resistance on insulin‐mediated peripheral glucose uptake have the same sensitivity for the insulin‐mediated suppression of ketogenesis. This implies differential insulin sensitivity of intermediary metabolism in obesity.

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A method is described for measuring pentachlorophenol (PCP) in samples from the estuarine environment. Gas-liquid chromatography (GLC) is used to determine PCP residues in tissues as low as 0.01 ppm by formation of the ethyl diazohydrocarbon derivative, followed by Florisil cleanup. Application of the method to exposed organisms indicates that PCP accumulates in mullet (Mugil cephalus), grass shrimp (.Palaemonetes pugio), and eastern oysters (Crassostrea virginica). Sea water concentrations as low as 0.002 ppb may be detected by formation of the amyl diazohydrocarbon derivative. Formation of the amyl derivatives of PCP and several related compounds gives GLC separation not possible with the methyl or ethyl derivatives. Parameters are outlined for high-pressure liquid chromatography (LC) determination of the free phenol without cleanup. Ultraviolet detection limits for PCP by LC are 5.0 ppm in tissues and 2.0 ppb in seawater.Pentachlorophenol (PCP) is a herbicide, fungicide/ bactericide, and insecticide that, together with its salts, has a broad spectrum of industrial, agricultural, and domestic applications (Benvenue and Beckman, 1967). U.S. production of PCP in 1977 was expected to be 80 million pounds (Cirelli, 1978), and annual Canadian usage