Patterns of inheritance of the chloroplast genome in Passiflora were analyzed by examining the progeny from both interspecific and intraspecific crosses. Artificial crosses of field-collected material were performed in greenhouses at The University of Texas at Austin. DNA from fresh leaf material was analyzed by Southern blot techniques to identify the donor of the chloroplast genome. Initially, single progeny were analyzed for 11 crosses; two intraspecific crosses demonstrated maternal inheritance, whereas the nine interspecific crosses had paternal inheritance. Subsequently, the donor of the chloroplast genome was determined for multiple progeny in seven crosses. Passiflora oerstedii × P. retipetala showed strict paternal inheritance in all of 17 progeny. A series of five crosses and backcrosses between P. oerstedii and P. menispermifolia demonstrated strictly paternal inheritance. Finally, when 15 progeny were analyzed for the P. costaricensis × P. costaricensis cross, 12 of the 15 showed maternal inheritance, whereas the remaining three were biparental. Interestingly, all interspecific crosses had primarily paternal inheritance, whereas all intraspecific crosses had primarily maternal inheritance. The implications of heteroplasmy on phylogenetic analyses of chloroplast DNA are discussed.

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JSTOR is a not-for-profit service that helps scholars, researchers, and students discover, use, and build upon a wide range of content in a trusted digital archive. We use information technology and tools to increase productivity and facilitate new forms of scholarship. For more information about JSTOR, please contact support@jstor.org.. American Society of Plant Taxonomists is collaborating with JSTOR to digitize, preserve and extend access to Systematic Botany.ABSTRACT. Two new species, Passiflora amoena and Passiflora callistemma, were discovered during a taxonomic revision of subgenus Astrophea. The first is placed in section Botryastrophea, the second in section Astrophea. A key is provided for all the species of Passiflora subgenus Astrophea.

Systematic and ecological implications were shown for Australian seagrasses by studies of isozymes, secondary compounds and experimental cultures. In Halophila, small- and large-leaved plants which occur sympatrically from northern Queensland to New South Wales and were referred to H. ovalis (R.Br.) Hook. f. differed in isozymes and secondary compounds and maintained leaf size differences in experimental cultures. The small-leaved plants should be referred to a different species. In Halodule, narrow-leaved plants that were referred to H. uninervis (Forsk.) Aschers. and H.pinifolia (Miki) Den Hartog were examined. In experimental cultures, narrow-leaved plants from Thursday Island produced both tridentate and rounded serrulate leaf tips and differed in isozymes and secondary compounds from wide-leaved plants. The narrow- leaved plants should be referred to H. pinifolia and the wide-leaved plants to H. uninervis. In Zostera, small- and large-leaved forms of Z. capricorni Aschers. which occur sympatrically in Queensland and New South Wales have similar isozymes that differ from those of Z. muelleri Irmisch. ex Aschers. of South Australia. Experimental cultures of Z. capricorni continued to produce 5-nerved leaves and those of Z. muelleri, 3- nerved leaves. In Amphibolis, cultures of A. antarctica (Labill.) Sonder & Aschers. and A. griffithii (J. M. Black) Den Hartog continued to show leaf differences but were similar in secondary compounds and isozymes, differing in only one of eight enzyme systems. In Posidonia, wide-leaved plants of P. australis Hook. f. continued to produce leaves in culture that differed in width from those of P. sinuosa Cambridge & Kuo but were similar in secondary compounds and isozymes, differing only in one of eight enzyme systems. The chemical evaluations and experimental cultures suggested wide differences in Halophila and in Halodule but indicated only narrow differences in the other three genera.