Background: LMBD1 is a transmembrane protein encoded by the lmbrd1 gene. Its biological function remains unclear. Results: lmbrd1 knockdown hinders the internalization of the insulin receptor, allowing it and its downstream signaling molecules to remain activated. Conclusion: LMBD1 functions as an insulin receptor-specific adaptor for endocytosis. Significance: LMBD1 is the first identified protein that participates in the regulation of insulin receptor endocytosis.

The results suggested that Lmbrd1 gene not only plays a significant role in mediating the energy homeostasis in cardiac tissue, it may also be a key factor in the regulation of cardiac function in mice.