In this study, we developed and validated a new analytical method for the quantitative evaluation of uric acid in delactosed permeate using ion chromatography coupled with an ultraviolet detector. Uric acid was extracted from delactosed permeate samples by diluted hydroxide solution at room temperature. The separation was carried out on a high‐capacity anion‐exchange column by isocratic elution with 50 mM potassium hydroxide. Uric acid was detected at 295 nm. The results from this study demonstrate that the new ion chromatography‐ultraviolet method facilitates both the resolution and the detection of uric acid and provides a highly accurate and sensitive determination of uric acid in delactosed permeate samples with minimal sample preparation effort. The new method provided an excellent range of analyte response linearity in the 2.5–255.6 ng range with R2 > 0.99. The limit of detection for uric acid obtained was 0.04 mg/kg in 1 mM sodium hydroxide. Intraday and interday variabilities were less than 3%, and the mean recoveries (%) of the uric acid spiked in two types of delactosed permeate were between 100.4% and 103.9%. This method was successfully applied to determine uric acid amounts in ten delactosed permeate samples.

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