In this work, methyl jasmonate (MeJA), as an exogenous elicitor, was used to investigate the feasibility of enhancing triterpenoid biosynthesis in Inonotus baumii. The results showed that the appropriate concentration of MeJA to be added was 150 mmol/L, and the triterpenoid yield was 12.61 mg/g dry weight (DW), which was 4.05-fold higher than that in the control with water. Moreover, quantitative real-time polymerase chain reaction was performed to measure the transcript levels of several genes in the triterpenoid pathway, including those encoding hydroxy-3-methylglutaryl-Coenzyme A synthase (hmgs), hydroxy-3-methylglutaryl-Coenzyme A reductase (hmgr), farnesyl pyrophosphate synthase (fpps), squalene synthase (sqs), squalene epoxidase (se) and lanosterol synthase (ls). The results demonstrated that these genes were mostly up-regulated by MeJA, although the levels of induction differed. For hmgs, hmgr, fpps and se, the highest transcript levels were at 150 mmol/L MeJA, and were approximately 3.0, 2.5, 2.0 and 2.0-fold higher than those in the control, respectively. However, the sqs and ls genes appeared to be repressed by MeJA. To the best of our knowledge, this is the first time to assess the use of MeJA to elicit triterpenoid biosynthesis in I. baumii, and the results indicated that MeJA was indeed a potent inducer of triterpenoid biosynthesis.
The cDNA encoding a-L-arabinofuranosidase was cloned from the edible fungus Auricularia auricula for the first time. The open reading frame of the a-L-arabinofuranosidase gene abf was 1953 bp encoding 650 amino acids, with a predicted protein molecular weight of 71.19 kDa and a theoretical isoelectric point of 5.23. The putative protein was predicted to belong to the glycoside hydrolase family-51. In addition, abf was cloned into the pET-32a vector and then expressed in Escherichia coli BL21. The recombinant protein, with an expected molecular weight, was observed in sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Moreover, the transcription levels of abf in response to different carbon sources were investigated in this study. The results showed that the expression of abf was mostly up-regulated when the mycelia were grown in different carbon sources, and L-arabinose or maltose induction had a significant effect on the expression of abf, which was 5.13-and 4.58-fold higher than that in the untreated control sample, respectively. In addition, the highest transcript levels induced by glucose and sucrose appeared on the third day and the levels were 2.47-and 3.11-fold higher compared to the control. These results laid a foundation for further studies on the a-L-arabinofuranosidase from A. auricula.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Copyright © 2025 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.