A cheap, small, convenient, reliable, and durable lab‐on‐a‐chip is needed for various scenarios in practical applications. The authors hope to use the chip only after plugging in a mobile phone. An ideal micropump design is obtained by simplifying the micropump theory, reducing the micropump to 1 mm (oscillator pump, O‐pump), the external device (actuator) to 5 mm, and the power to 10 mW. This system costs ≈$10 and can work in harsh fluid environments (such as animal cell culture) for an extended period (a few months of testing). An MP3 player drives two O‐pumps by a programmable playlist. The O‐pump is a permanent magnet positive sphere in an open fluid space, which is not easy to block and rolls freely without wear. Only controlled by the magnetic field, the O‐pump avoids interference from the current, electric field, and chemical activity. The fluid is driven by a vibrating motion, in open or sealed channels, with no unique materials and no other equipment. The debris, bubbles, and fibers in the blood viscosity fluid do not affect the pump's function. The system can be applied in different scenarios, such as the automatic cultivation of stem cells or point‐of‐care devices.

This article describes a technology to transform a petri dish into a microfluidic chip with a stem cell nest for stem cell niche engineering. A permanent magnet sphere is put in a 30 mm petri dish as an oscillator pump (O‐pump). An earphone‐sized actuator outside the petri dish receives the programmed audio signal from an MP3 player or a mobile phone to drive the in‐dish O‐pump for a microflow. There are guiding walls to form a wake area for the cell nest at the dish's center. The cell nest uses the wake to create a stable, ultraslow internal microcirculation flow. The retention half‐life of nanoparticles in the cell nest is 1419.8 ± 1.3 s. By setting the microfluidic program and supplementing five drops of the culture medium outside the cell nest every 2 d, the growth rate of embryonic stem cells in the cell nest during the 8 d automatic culture appears significantly optimized. The single clone area of embryonic stem cells increases from (3.57 ± 0.52) × 105 to (11.67 ± 1.33) × 105  µm2  after 2 d of the automatic culture and advances to (53.34 ± 8.37) × 105  µm2 after 4 d.