The ability of Trichoderma viride to synthesize extracellular enzymes (Lacase enzyme) using a substrate that contains media was examined. This isolate generated laccase at its greatest level (0.166U/ml) in liquid medium.  This study was  demonstrated the production, purification, and characterisation of the laccase enzyme from T.viride. The results showed that 96 hours was the optimum period of time for Laccase to incubate from this fungus. Laccase displayed the maximum level of activity at pH 4.5 and temperature 30 °C. The results revealed that the best ratio for laccase precipitation was 90%  by using ammonium sulphate.  In addition, for the purification of laccase enzyme, one peak of Laccase was appeared  in gel filtration purified from T.viride, while 2 peaks of Laccase were seen in ion exchange chromatography. According to the findings, the molecular weight of T. viride Laccase using SDS-PAGE was roughly 66KD under denaturation conditions.

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Nanotechnology is quickly becoming one of the most essential and transformative areas of science. Physical, chemical, mechanical, and biological approaches are all used to create nanoparticles. Plants or microorganisms are frequently used in biological methods of metal ion reduction because they are clean, nontoxic, safe, biocompatible, and ecologically friendly. Fourir transform infra-red spectroscopy (FT–IR), scaning electron microscopy (SEM), and X-ray diffraction spectroscopy were described to analyze the nanoparticles generated (XRD). Nanoparticles (NPs) made from fungi have a diverse range of bio catalytic techniques, including enzyme immobilization for increased enzymatic activity. Silver (Ag) NPs made from fungi were discovered to have a benign effect in a wound and a thermal wound, and to have anti-mosquito, antibacterial, and antifungal properties