Chestnuts are popular edible nuts that are rich in starch. In order to enhance the transcriptomic resources and further understand starch and sucrose metabolism in maturing chestnuts, a comparative transcriptomic study of Chinese chestnut kernels was conducted at three ripening stages (70, 82, and 94 DAF). At 82 and 94 days after flowering (DAF), starch continued to accumulate, and the amylopectin/amylose ratio increased. Transcriptomic profiling of kernels at 70 (stage I), 82 (stage II), and 94 DAF (stage III) indicated that soluble starch synthase and α-1,4-glucan branching enzyme genes are actively expressed at 82 and 94 DAF. The starch degradation enzymes amylase, phosphoglucan phosphatase DSP4, and maltose exporter did not show differential gene expression, while glycogen phosphorylase-encoding unigenes were significantly down-regulated at 94 DAF. In addition to starch and sucrose metabolism, RNA transport, RNA degradation, pyrimidine metabolism, purine metabolism, plant hormone signal transduction, plant–pathogen interactions, and glycerophospholipid metabolism were found to be significantly enriched in all comparisons included in the study. As Chinese chestnut matured, the unique enriched pathways switched from ribosomal biogenesis and RNA polymerase of eukaryotes to endocytosis and spliceosomes. These genomic resources and findings are valuable for further understanding starch and sucrose metabolism in the Chinese chestnut.

No abstract

The box-plate steel structure residence is a box structure with stiffened steel plates directly used as load-bearing walls and floors. In practical engineering, due to the functional requirements of the building, it is necessary to open door or window openings on the box-plate steel structure walls. To study the seismic performance of the box-plate steel structure with openings system, two three-story single-compartment box-plate steel structures with openings modular units were designed and fabricated according to the 1:3 reduced scale. Through the quasi-static loading test, numerical simulation, and theoretical analysis, the failure process, failure mode, lateral force resistant capacity, and hysteresis performance of the specimens were studied. The impact of the different opening areas and opening position on the seismic performance of the box-plate steel structure was emphatically analyzed. The results of the test indicated that the openings on the steel wall plate would reduce the initial stiffness and the lateral force resistant capacity of the specimen; the destruction of the box-plate steel structure with openings modular unit under the low cyclic loading effect started with the tear in the corner of the openings and ended with the tear in the corner steel wall plate. Then, the finite element analysis (FEA) models were developed to supplement the experimental study, and the comparisons were made between measured and simulated results on load versus displacement relationships and failure modes. On the basis of the stressing mechanism of the box-plate structure modular unit, the calculation equation of the lateral force resistant capacity of the box-plate structure with openings modular unit was put forward. Then, the proved finite element analysis (FEA) models were used for parameter analysis of different influence parameters to verify the proposed calculation equation. The results showed that the proposed calculation equation had high accuracy and could be used as a design basis for practical engineering.

No abstract