Due to their pathogenic potential, identifying Vibrio species from recirculating aquaculture systems (RAS) for Pacific white shrimp (Litopenaeus vannamei) is of great importance to determine the risk for animal's as well as for the consumer's health. The present study compared identification results for a total of 93 Vibrio isolates, including type strains and isolates from shrimp aquaculture. Results from biochemical identifications, 16S rRNA sequencing, sequencing of the uridylate kinase encoding gene pyrH and analysis of the protein spectra assessed by MALDI‐TOF MS were compared. The results achieved by these different methods were highly divergent for many of the analysed isolates and for several Vibrio spp difficulties in reliably identifying occurred. These difficulties mainly resulted from missing entries in digital databases, a low number of comparable isolates analysed so far, and high interspecific similarities of biochemical traits and nucleotide sequences between the closely related Vibrio species. Due to the presented data, it can be concluded that for identifying Vibrio spp. from samples in routine diagnostics, it is recommended to use MALDI‐TOF MS analysis for a quick and reliable identification of pathogenic Vibrio sp. Nevertheless, editing the database, containing the main spectra of Vibrio is recommended to achieve reliable identification results.

Pacific white shrimp (Litopenaeus vannamei) are one of the most frequently cultivated shrimp species worldwide (Ezquerra Brauer, Salazar Leyva, Bringas Alvarado, & Rouzaud Sández, 2003). Their native habitat is the eastern Pacific coast of South America with an average water temperature of above 20°C and a salinity of 34-37‰. In aquaculture, these shrimps are produced in extensive pond culture at water temperatures between 26-32°C in large quantities, mostly in South America and Asia (Bundesverband Aquakultur Garnele, 2018; FAO). By developing land-based recirculating aquaculture systems (RAS), the production of L. vannamei has become

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