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The specijcity of complement antiglobulin agglutinationEvidence was presented in a previous report [14] that the agglutinogen of the so-called "non-gamma globulin" antiglobulin reaction [ l , 2, 16, 151 was dependent upon complement or a protein material fixed nonspecifically in the presence of complement. These observations corroborated earlier work [3] but demonstrated that the sensitivity of agglutination was increased with the use of antisera to human complement. I n addition it was shown that EAC' (optimally sensitized sheep cells complexed with C') could be used as an experimental tool and also as a means of reagent assay.Because of the complexity of C', experiments using total complement activity and specific component activity were designed to elucidate the antiglobulin specificity of antisera to complement. These experiments were based on the current concept of the reaction mechanism of immune hemolysis with guinea pig serum. Investigations of Pillemer [ll] and Leon [6] have shown that a similar reaction mechanism is involved when human serum is the source of complement. Differences were known to exist and some were encountered in the course of this work. These differences were studied and the results are being reported separately [12, 131.

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