The present study aimed to verify the unique effects of sex, grade and school, as well as interactive effects (sex vs grade), on enjoyment of Physical Education. The sample was composed of 301 students (140 males and 161 females). The Brazilian version of Physical Education Teaching Processes Questionnaire was applied in the classroom. The results generally indicated that sex, grade and school have a unique significant effect in the on enjoyment of Physical Education. Furthermore, interactive effects (sex vs grade) were noticed. Boys who attend less advanced grades tend to report higher levels of enjoyment in school Physical Education. A school Physical Education class that respects the interests and needs of students and which is concerned with motivating its students to succeed in Physical Education at school may encourage them to be physically active outside of school.