ObjectiveTo evaluate the Knoop hardness, composition, and wear resistance of acrylic-resin artificial teeth exposed to mechanical toothbrushing. Methods Artificial under a 200-g load and at a frequency of 250 cycles per minute, using a soft-bristled toothbrush (IndicatorPlus 30, Oral-B) soaked in a 1:1 toothpaste/water slurry (Oral B Pró Saúde). Microhardness testing was performed using a 25-g load for 15 seconds in an HMV-2 hardness tester (Shimadzu). The composition of teeth from different brands was determined by scanning electron microscopy with energy-dispersive X-ray spectroscopy (SEM/EDS) (Jeol JSM 5800). Results Wear results after mechanical brushing were compared by means of the paired t-test, whereas those obtained in microhardness testing were compared by ANOVA with Bonferroni correction. There was no statistically significant difference between brands in either trial. ConclusionComposition analysis revealed that all of the artificial teeth analyzed contain carbon and oxygen. Trilux and Soluut PX brand teeth also contain silicon; however, the presence of filler particles did not result in increased resistance.