Delineating the spatial multiomics landscape will pave the way to understanding the molecular basis of physiology and pathology. However, current spatial omics technology development is still in its infancy. Here, we developed a high-throughput multiomics in situ pairwise sequencing (MiP-Seq) strategy to efficiently decipher multiplexed DNAs, RNAs, proteins, and small biomolecules at subcellular resolution. We delineated dynamic spatial gene profiles in the hypothalamus using MiP-Seq. Moreover, MiP-Seq was unitized to detect tumor gene mutations and allele-specific expression of parental genes and to differentiate sites with and without the m6A RNA modification at specific sites. MiP-Seq was combined with in vivo Ca2+ imaging and Raman imaging to obtain a spatial multiomics atlas correlated to neuronal activity and cellular biochemical fingerprints. Importantly, we proposed a signal dilution strategy to resolve the crowded signals that challenge the applicability of in situ sequencing. Together, our method improves spatial multiomics and precision diagnostics and facilitates analyses of cell function in connection with gene profiles.

As humanised design served for the idea of "social responsibility", this research takes a sociological survey on humanised design of in-patient departments of public hospital, as a case study, in order to present an example to value the significance of sociological survey upon reliable design. With well planned research target and communications, we take the survey methods as direct observation, questionnaire and group interview to obtain necessary information about needs of concerned people. Then, a series of design problems was found and the corresponding suggestions were made for improving solutions based upon comparative analysis. Finally, it reveals the strong relationship between social survey and improvement of humanised communication design with social responsibility.