We aimed to verify the reproducibility of the results obtained from previous systematic reviews and meta-analyses, focusing specifically on methodological and technical aspects, such as navigation method, screw insertion technique and spinal region involved to provide a more homogenous analysis. The PubMed database was searched using three terms: “stereotaxic techniques”, “neuronavigation”, and “image-guided surgery,” each associated with other two terms, “pedicle” and “screw”. Data were transferred to Comprehensive Meta-Analysis software, v.3, for statistical analysis. The significance of the pooled odds ratio was determined by the Z test, along with95% confidence intervals. A funnel plot was used to assess publication bias. The statistical significance was defined as p ≤ 0.05. The meta-analysis yielded an overall odds ratio of 2.9 favouring navigation. The average correct positioning of the screws was 91.8% for navigation, while 82.2% of screws were deemed appropriate in fluoroscopy. The odds ratio obtained for the thoracic and lumbar spines separately was 3.1 and 2.7, respectively. Among the fluoroscopy guided surgeries, 53 complications occurred, whereas only eleven complications were observed among those guided by neuronavigation. This meta-analysis suggests that compared with conventionalfluoroscopy technique, neuronavigation allows for more accurate pedicle screw placement.