Abstract

A number of chemical, microbial, and eukaryotic indicators have been proposed as indicators of fecal pollution sources in water bodies. No single one of the indicators tested to date has been able to determine the source of fecal pollution in water. However, the combined use of different indicators has been demonstrated to be the best way of defining predictive models suitable for determining fecal pollution sources. Molecular methods are promising tools that could complement standard microbiological water analysis. In this study, the feasibility of some proposed molecular indicators for microbial source tracking (MST) was compared (names of markers are in parentheses): host-specific Bacteroidetes (HF134, HF183, CF128, and CF193), Bifidobacterium adolescentis (ADO), Bifidobacterium dentium (DEN), the gene esp of Enterococcus faecium, and host-specific mitochondrial DNA associated with humans, cattle, and pigs (Humito, Bomito, and Pomito, respectively). None of the individual molecular markers tested enabled 100% source identification. They should be combined with other markers to raise sensitivity and specificity and increase the number of sources that are identified. MST predictive models using only these molecular markers were developed. The models were evaluated by considering the lowest number of molecular indicators needed to obtain the highest rate of identification of fecal sources. The combined use of three molecular markers (ADO, Bomito, and Pomito) enabled correct identification of 75.7% of the samples, with differentiation between human, swine, bovine, and poultry sources. Discrimination between human and nonhuman fecal pollution was possible using two markers: ADO and Pomito (84.6% correct identification). The percentage of correct identification increased with the number of markers analyzed. The best predictive model for distinguishing human from nonhuman fecal sources was based on 5 molecular markers (HF134, ADO, DEN, Bomito, and Pomito) and provided 90.1% correct classification.

The control and prediction of wastewater treatment plants poses an important goal: to avoid breaking the environmental balance by always keeping the system in stable operating conditions. It is known that qualitative information -coming from microscopic examinations and subjective remarks -has a deep influence on the activated sludge process. In particular, on the total amount of effluent suspended solids, one of the measures of overall plant performance. The search for an input-output model of this variable and the prediction of sudden increases (bulking episodes) is thus a central concern to ensure the fulfillment of current discharge limitations. Unfortunately, the strong interrelation between variables, their heterogeneity and the very high amount of missing information makes the use of traditional techniques difficult, or even impossible. Through the combined use of several methods -rough set theory and artificial neural networks, mainly -reasonable prediction models are found, which also serve to show the different importance of variables and provide insight into the process dynamics. ᭧

Abstract-In this paper, we present a detailed evaluation of a set of well-known Machine Learning classifiers in front of dynamic and non-deterministic software anomalies. The system state prediction is based on monitoring system metrics. This allows software proactive rejuvenation to be triggered automatically. Random Forest approach achieves validation errors less than 1% in comparison to the well-known ML algorithms under avaluation.In order to reduce automatically the number of monitored parameters, needed to predict software anomalies, we analyze Lasso Regularization technique jointly with the Machine Learning classifiers to evaluate how the prediction accuracy could be guaranteed within an acceptable threshold. This allows to reduce drastically (around 60% in the best case) the number of monitoring parameters. The framework, based on ML and Lasso regularization techniques, has been validated using an ecommerce environment with Apache Tomcat server, and MySql database server.