Aim:The purpose of this study was to investigate the effect of Erbium-doped yttrium aluminium garnet laser laser (Er: YAG laser), sandblast and several universal bonding on the shear bond strength of zirconia ceramic to composite resin. Materials and methods:In this experimental study, 96 Y-TZP disks were used. They were divided into six groups based on surface preparation: 1-Er: YAG laser + single bond universal/ 3M, 2-Er: YAG laser + all bond universal/bisco, 3-Er: YAG laser + G-premio bond/GC, 4-sandblast + single bond universal/3M, 5-sandblast + all bond universal/bisco, 6-sandblast + G-Premio bond/GC; in the next step, composite discs were cured on the surface of the zirconia discs and their shear bond strength was evaluated using a mechanical test machine (universal testing machine).Results: Two-way ANOVA showed that the surface preparation had a significant statistical effect on shear bond strength (p< 0.001). There was no association between these two variables with regards to the interaction of bonding and surface preparation (p = 0.064). Tukey's test showed that the shear bond strengths in the sandblast type group did not differ significantly between the groups according to the type of universal bonding, as well as in the Er: YAG laser treated group by type Universal bonding which was not significantly different between the groups of single bond universal and all bond universal, but there was a significant difference between the groups in terms of single bond universal and G-Premio, as well as all bond universal and G-Premio. Conclusion:Based on the results, the preparation of Er:YAG laser is a more appropriate method for increasing bond strength when compared with sandblasting, and among the universal bonding, G-Premio is also more suitable for this purpose. Clinical significance:The present data indicate that bond strength of laser preparation and G-Premio adhesive might be reliable for clinical application