The applicability of ionic liquids (ILs) has been increased during the last years and even new opportunities are becoming a reality, i.e. mixtures of pure IL and inorganic salt as electrolytes for smart electrochemical devices. In this work, the ecotoxicity of two protic ILs (Ethylammonium nitrate and Ethylimidazolium nitrate) and one aprotic IL (butylmethylpyrrolidinium bis(trifluoromethylsulfonyl)imide) doped with the corresponding Lithium salt was tested towards changes on the bioluminescence of the bacteria Aliivibrio fischeri, using the Microtox ® standard toxicity test. EC50 of these mixtures was determined over three standard periods of time and compared with the corresponding values to pure ILs.