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Background:The aim of the present study was to analyse the antidiabetic and antioxidant potential of GancidinW (GW) extracted from Streptomyces paradoxus VITALK03. Materials and Methods:Antidiabetic potential of GW was evaluated by assay of α-amylase and α-glucosidase inhibitory activity; haemoglobin glycosylation and yeast glucose uptake. The antioxidant potential of GW was assessed by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) assay, 2,2′azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation decolorization assay and superoxide assay. The inhibition of α-amylase and α-glucosidase by GW was also studied by in Silico molecular docking analysis. Results:GW (1mg/ml) showed 69.32% of α-amylase and 54.04% of α-glucosidase inhibitory activity. GW (1mg/ml) prevented haemoglobin glycosylation up to 30.92% and the glucose uptake by yeast cells was increased up to 64.38%. The binding interaction GW with αamylase showed the least free binding energy of -6.09Kcal/mol and -7.53Kcal/mol with α-glucosidase by docking studies. GW also demonstrated moderate antioxidant activity in all the antioxidant assays performed. Conclusion:The results of this study suggests that the antidiabetic and antioxidant potential of GW can be probed further to develop GW as effective antidiabetic agent.

Objective: The present study was performed to identify the phytochemical constituents of leaves and flowers of a plant Spermadictyon suaveolens extracted with four different solvents.Methods: Dried and powdered samples were subjected to soxhlation based on the polarity of the solvents. The extracts were scanned using Ultra Violet-visible (UV-Vis) spectrophotometry with the wavelength ranging from 200-800 nm by comparing the absorption spectrum with the spectra of known compounds, Fourier Transform Infrared (FT-IR) spectrometry was used to find out the functional groups of the compounds and GC-MS system consisting of a Perkin Elmer Technologies Model Clarus 680 GC equipped with Clarus 600 (EI) was used to identify the metabolites by matching their recorded mass spectra with the standard mass spectra from National Institute of Standards and Technology (NIST05. LIB) libraries provided by the software of the GCMS system (Turbo Mass version 5.4.2). Results:The phytochemical tests indicated the presence of carbohydrates, alkaloids, flavonoids, phenols, tannins, saponins and terpenoids from the chloroform extract of leaves and flowers. UV-visible spectrophotometer results indicated a wavelength range between 230-660 nm for the flower and leaf extracts for major peaks. FT-IR analysis indicated major functional groups such as aromatic, primary, secondary and aliphatic amines, alkanes, carboxylic acids and amides. GC-MS analysis results revealed major bioactive compounds in the crude extracts. Conclusion:Presence of secondary metabolites has been identified from the phytochemical studies. Many phyto-compounds have been identified from the leaves and flowers of using GC-MS analysis. Hence, this medicinal plant may be used as a source for treating many diseases.

Aim: This study was carried out to investigate the anticancer and antibacterial activity of date seeds and its phytochemical composition. Materials and Methods: Qualitative phytochemical screening was performed using date seed powder using biochemical assays. Gas chromatography-mass spectrometry (GC-MS) analysis was performed to identify the phytochemical contents in the extract. MTT assay was carried out to study the cytotoxicity of the seed extracts against HCT-15 (human coloretal cancer) cells. Antibacterial activity was studied using agar well diffusion method, against Bacillus cereus and Escherichia coli. Results: GC-MS analysis of acetone and chloroform extract of date seeds suggests that acetone extract contains majorly of aliphatic molecules and chloroform extract contains aromatic molecules. Cytotoxicity study showed that acetone extract is highly cytotoxic with inhibitor concentration 50 (IC50) value at 20 µg/ml and chloroform extract is moderately cytotoxic with IC50 value at 100 µg/ml concentration against HCT-15 cells. Antibacterial study showed that chloroform extract had no antagonistic activity against bacteria, whereas acetone extract demonstrated significant antibacterial activity with a zone of inhibition of 17 mm and 20 mm against B. cereus and E. coli, respectively at 1 mg/ml concentration. Conclusion: Results of this study conclude that acetone extract of date seeds (Phoenix dactylifera L) contains significant potential for pharmaceutical applications, in the field of antibacterial and anticancer drug discovery.