Of the five explants viz., shoot tip, nodal segment, leaf disc, cotyledon and root tip excised from exegenic seedlings of Citrus indica Tanaka shoot tip cultured in MS supplemented with 0.5 mg/l of BAP generated shoots within 4.74 days, exhibiting highest percentage of response (85.82%) with highest number of shoots (8.9) and shoot length (3.04 cm). On the other hand nodal segment cultured in MS supplemented with 1.0 mg/l BAP showed 80% response in 5.16 days with a shoot number of 5.41 and shoot length of 2.43 cm. Cotyledon explants inoculated on MS supplemented with1.0 mg/l of TDZ produced shoots in 20 days with the highest response of 69.88%, with 3.77 shoots per cotyledon and shoot length of 2.03cm. Viable callus was obtained from leaf disc cultured on half strength MS medium with less Ca++ with 2, 4-D 0.5 mg/l + Kn 0.25 mg/l. This callus when inoculated on half strength MS medium with Kn 1.5 mg/l showed highest shoot bud proliferation of 66.66% with 10.06 shoots per callus. Root tip explant failed to produce any shoots. In vitro raised shoots of Citrus indica when cultured on half strength MS medium supplemented with NAA (1.0 mg/l) showed 80 % rooting in 5.66 days, with highest number of roots (6.16 per shoot) and longest root (3.78cm). Ninety per cent of in vitro rooted plantlets of Citrus indica survived in open conditions. Plant Tissue Cult. & Biotech. 30(1): 87-96, 2020 (June)

A study was conducted to standardize a protocol for in-vitro direct regeneration and mass multiplication of Citrus indica Tanaka using shoot tip explants excised from mature trees. Shoot tips were inoculated in MS medium supplemented with varying concentrations and combinations of cytokinins and auxins. MS media when fortified with BAP 0.5 mg/l and 0.5 mg/l BAP + 1.0 mg/l Kn were found to be the best treatments for shoot initiation while MS supplemented with 1 mg/l IBA; 0.5 mg/l NAA + 0.5 mg/l IBA and 0.5 mg/l NAA + 0.5 mg/l IAA were the best treatments for root induction. Among the different media used for hardening, 100 % survivability was obtained when plantlets were hardened using vermicompost as the potting medium. Subsequently, these plantlets were transferred to larger pots and acclimatization was achieved gradually in outdoor conditions. Plant Tissue Cult. & Biotech. 31(1): 13-23, 2021 (June)

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