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The turbulence moment of order m (μm) is defined as the refractive index structure constant Cn2 integrated over the whole path z with path-weighting function zm. Optical effects of atmospheric turbulence are directly related to turbulence moments. To evaluate the optical effects of atmospheric turbulence, it is necessary to measure the turbulence moment. It is well known that zero-order moments of turbulence (μ0) and five-thirds-order moments of turbulence (μ5/3), which correspond to the seeing and the isoplanatic angles, respectively, have been monitored as routine parameters in astronomical site testing. However, the direct measurement of second-order moments of turbulence (μ2) of the whole layer atmosphere has not been reported. Using a star as the light source, it has been found that μ2 can be measured through the covariance of the irradiance in two receiver apertures with suitable aperture size and aperture separation. Numerical results show that the theoretical error of this novel method is negligible in all the typical turbulence models. This method enabled us to monitor μ2 as a routine parameter in astronomical site testing, which is helpful to understand the characteristics of atmospheric turbulence better combined with μ0 and μ5/3.