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Optimal conditions for demonstrating the presence of infectious human immunodeficiency virus in peripheral blood mononuclear cells (PMCs) from seropositive individuals involved cocultivation of infected cells with phytohemagglutinin-stimulated PMCs from seronegative donors in the presence of 2 ,ug of Polybrene per ml. The size of the culture vessel also influenced the results; smaller numbers of infected cells were detected under conditions of increased cell density. In addition, an increased normal donor/patient PMC ratio was helpful. The cocultivation approach permitted identification of human immunodeficiency virus in over 90% of seropositive individuals with different clinical conditions. Moreover, reconstruction experiments indicated that this method allows detection of one productively infected CD4+ cell in a population of over 106 PMCs.

Supplement'') was developed in partnership with a multidisciplinary panel of board-certified physician and doctoral experts in the fields of infectious disease, immunology, public-health policy, dermatology, facial plastic surgery, and plastic surgery. The Guidance Supplement is intended to provide aesthetic medicine physicians and their staff with a practical guide to safety considerations to support clinic preparedness for patients seeking nonsurgical aesthetic treatments and procedures following the return-to-work phase of the coronavirus disease 2019 (COVID-19) pandemic, once such activity is permitted by applicable law. Many federal, state, and local governmental authorities, public-health agencies, and professional medical societies have promulgated COVID-19 orders and advisories applicable to health-care practitioners. The Guidance Supplement is meant to provide aesthetic physicians and their staff with an additional set of practical considerations for delivering aesthetic care safely and generally conducting business responsibly in the new world of COVID-19. The Guidance Supplement is published as an Appendix to this article and can be read in full online at www.liebertpub.com/fpsam Aesthetic providers will face new and unique challenges as government stay-at-home orders and related commercial limitations are eased, the U.S. economy reopens, and health-care systems transition from providing only urgent and other essential treatment to resuming routine care and elective procedures and services. Medical aesthetic specialties will therefore wish to resume practice in order to ensure high-quality expert care is available and, importantly, to help promote patients' positive self-image and sense of well-being following a lengthy and stressful period of quarantine. In a number of areas, this Guidance Supplement exceeds traditional aesthetic office safety precautions, recognizing reduced tolerance in an elective treatment environment for any risk associated with COVID-19's highly variable presentation and unpredictable course. The disease has placed a disturbing number of young, otherwise healthy patients in