The common bed bug (Cimex lectularius) has been a persistent pest of humans for thousands of years, yet the genetic basis of the bed bug's basic biology and adaptation to dense human environments is largely unknown. Here we report the assembly, annotation and phylogenetic mapping of the 697.9-Mb Cimex lectularius genome, with an N50 of 971 kb, using both long and short read technologies. A RNA-seq time course across all five developmental stages and male and female adults generated 36,985 coding and noncoding gene models. The most pronounced change in gene expression during the life cycle occurs after feeding on human blood and included genes from the Wolbachia endosymbiont, which shows a simultaneous and coordinated host/commensal response to haematophagous activity. These data provide a rich genetic resource for mapping activity and density of C. lectularius across human hosts and cities, which can help track, manage and control bed bug infestations.

Although no members of the spider family Prodidomidae have previously been described from Chile, that country has a substantial, and largely endemic, prodidomid fauna. The Argentine genus Moreno Mello-Leitao is transferred from the Gnaphosidae to the Prodidomidae.Moreno was previously known only from the female holotype of its type species, but two additional species from Argentina and three from Chile are newly described, including the first known males of the genus. Three new genera are described: Chileomma, containing seven new six-eyed species from northern and central Chile; Chilongius, containing five new species from northern Chile; and Chileuma, containing three new species from northern and central Chile. A newly discovered feature on the tarsal claws is hypothesized to be a synapomorphy uniting Moreno and Chilongius with the other South American tricongiine Theuminae.

It has been well documented, worldwide, that inhalation and/or contact with airborne particulate insect products has resulted in sensitivity to insect proteins and is manifested by such common entities as dermatitis, conjunctivitis, rhinitis, and asthma. However, the deliberate ingestion of a variety of insects (undertaken to prove their edibility and nutrient value) resulted in subsequent sensitization of some individuals. Such an outcome has not previously been reported in the literature. The objective was to document the anaphylactic reaction to the purposeful ingestion of mealworm in an individual known to be sensitized to the inhalation of beetle larvae. We used the occasion of the Centennial Celebration of The New York Entomological Society to expose members and guests of the Society to the ingestion of various insects. The subjects of the study consisted of: 1) Three members were adversely affected; 2) One individual with Baker's asthma; and 3) A number of controls with no known hypersensitivity to insect products. The investigation was undertaken by food challenges, inhalation challenges, skin testing to the individual insect allergens, a) Tenebrio molitor (TM), b) Zophobas morio (ZM), c) Blattella germanica (BG), skin testing to common indoors and outdoor allergens, and direct bind ELISA and ELISA inhibition. One individual manifesting hypersensitivity both by ingestion and inhalation to mealworm was identified. This sensitivity was documented clinically as well as by objective testing.

The Canadian Entomologist 125: 931-944 (1993) The family Acroceridae (Tnsecta: Diptera; "Small Headed Flies") are a seldom seen yet cosmopolitan group of endopansitoids of spiders. Recent host and distribution records are presented here for six species of acrocerids: Ogcodes bnreulis Cole. 1919; Ogcoir'es pu/lidipennis (Loew. 1866); Ogcodes sp. ; Acroceru bimucularu Loew. l8*, Turbopsebiur srtlphuripes (loew, 1869); and Exetasis eickstedtae Schlinger, 1972. New hosts for each fly species are: 0. borealis-Schizocosa rovncri Uetz and Dondale, 1979, Pardusa spp. ; 0. pul/idipenni.~4chizocosu rovneri, Schizocnsu spp.; Ogcodes sp.-Anyphaena californica (Banks, 1904); Acrocera bimaculata--Coras montanus (Emerton, 1890b); T. sulphuripes-4. montanus.Detailed field measurements and behavioral observations of host spiders and fly development are described and compared with known data. Examination of these comparisons suggests that host-parasitoid relationships follow spider guild aqsociations (i.e. groundlsurface dwelling hosts or those building webs in close contact with surfaces), especially with the spider family Agelenidae. These affiliations probably result from a combination of the spider's web building. web maintenance, hunting hehaviors. and fly oviposition activities, which dispose spiders exhibiting these hehaviors to greater chances for parasitoidism. These factors act in concen to increase probabilities for host-parasitoid interactions. Compiled data indicate duration of pupation may be related to ambient temperature. Evidence is presented that acrocerid larvae may alter their hosts' behavior to increase the parasitoids' probability of survival. Cady, A., R. Leech, L. Sorkin, G. Stratton et M. Caldwell. 1993. Les Acroceridae (Insecta: Diptera): cycles biologiques, comportements, araignkes hBtes et rkpartitions gkographiques. The Canadian Entomologist 125: 931-944. Resume La famille des Acroceridae (Insecta: Diptera; "mouches a petite tEte") constitue un groupe d'insectes rarement vus et pourtant cosmopolites, parasites d'araignkes. On trouvera ici une liste de donnCes rkcentes sur les hBtes et les rCpartitions de six espkces d'acrockres: Ogcodes borealis Cole, 1919, Ogcodes pallidipennis (Loew, 1866), Ogcodes sp., Acrocera bimaculata Loew, 1866, Turbopsebius sulphuripes (Loew, 1869) et Exetasis eickstedtae Schlinger, 1972. Pour chacune de ces esp&ces, les nouveaux hBtes rencontrks sont: dans le cas d'O. borealis, Schizocosa rovneri Uetz et Dondale, 1979 et Pardosa spp., dans le cas d'O. pallidipennis. Schizocosa rovneri et Schizocosa spp., dans le cas d'ogcodes sp., Anyphaena californica (Banks, 1904), dans le cas d'Acrocera bimaculata, Coras montanus (Emerton, 1890b) et dans le cas de T. sulphuripes, C . montanus.Des mesures detaillees et des observations du comportement des araignees h8tes recueillies sur le terrain sont presentees ici et comparees aux donnees de la litterature. Les comparaisons indiquent que les relations h8tes-parasito'ides se font en fonction des guildes d'araignees (i-e. hBtes habitan...

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