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Harris (19504. The data obtained for a number of foods indicated they had exceptionally high nutritive values and could make significant contributions to the nutritive value of the diet.Results from analyses of 51 samples collected in Eicaragua in November, 1946, and of four samples shipped from Panama, are shown in the present report. The samples from Nicaragua included 40 of fresh foods, 10 of dry cereals and legumes, and one classified as miscellaneous; those from Panama, three of citrus fruits and one of a nut.The fresh samples were stabilized by the procedure already described, Munsell et al. (1949), and shipped by air express to Cambridge, where the analytical work was done. Two slurries only were made of each sample a t this time of the study. Subsamples of the citrus fruits from Panama were stabilized in Honduras for final shipment. The dry samples were packed in tight metal containers for shipment. The same constituents were measured as shown in the preceding reports and methods of analyses used were the same as described in the first paper, Munsel e t al. (1949), except for the determination of ascorbic acid in the three samples of citrus fruits from Panama. For these analyses the modified method described in the second report, Munsell et al. (1950a), was used.Values obtained from the analyses and the places of origin of the samples are shown (Table 1). The majority of samples from Nicaragua originated in the western coastal-to-highland area, where the altitude ranges from 200 to 2,000 feet and the soil is predominantly volcanic. No estimates of the annual rainfall were obtained. All except three of the samples were purchased in markets or local stores. These three samples, ayote, sandia, and zapote amnrillo, were taken directly from plants. The 51 samples from Nicaragua represent 42 foods; there were more than one sample €or only four of these. 355

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A description of the procedure followed in the collection and preparation of samples for shipment, and an outline of the information recorded on the accompanying data sheets, were given in the first report of this series. Kodachromes were taken in duplicate of all samples, as has been done throughout the study; one set to be kept on file a t the Escuela Agricola Panamericana and the other in the M. I. T. Nutritional Biochemistry Laboratories. Specimens were also taken for the herbarium at the Escuela.Methods of analysis used have been described in Reports I and 11. The same 13 constituents were measured as shown in the first seven papers.Of the 79 samples analyzed, 78 were purchased in markets, 73 in the several markets in Guatemala City, and five in Antigua. The remaining sample, apio, was taken from the ground at the Escuela Nacional de Agricultura near BBrcena. Information on altitude, prevailing soil type, and estimated annual rainfall in the areas where samples originated is summarized i n Table 1. It should be noted that the majority of the samples came from the highland areas, where the soil is of volcanic origin.