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Introduction: Increased Vascular Endothelial Growth Factor A (VEGF-A) levels are associated with Severe Acute Respiratory (SARS) infection. The aim was to investigate in vivo VEGF-A and VEGF-B (VEGF-A/B) gene expression (GE) in severe pulmonary disease pathogenesis. Method: Twelve temporal Mus musculus Wildtype (WT) C57BL/6 SARS-CoV MA15 lung studies were selected from the NCBI GEO database for GE profiling. Results: In murine dataset (GSE68820) Day 2 was compared to Day 7 demonstrating a downregulation trend in VEGF-A GE, with an opposite effect on VEGF-B GE (p=4.147e-03, p=7.580e-07, respectively). A v-shaped VEGF-B gene expression trajectory was noteworthy across certain datasets and after dORF6 stimulation. In addition, MA15 dose stimulation studies showed that a higher antigenic load caused more profound effects on VEGF-A resulting in a steeper fall in GE compared to other antigens. Conclusions: Distinct temporal trajectory patterns of VEGF-A and VEGF-B gene expression were associated with SARS-CoV MA15 stimulation. Unraveling the importance of VEG-A/B dynamics offers exciting prospects for improved bio-marking and therapeutic precision.

Polygenic Risk Score (PRS) models are used extensively to find the population/individual risk towards disease. These predictive scores are of great help as risk scores if predicted earlier the life of individual can be saved from the chronic/ complex diseases. In this empirical assessments study, the polygenic risk score was calculated in three different ancestries (SAS, EAS and African Americans) based on more than three hundred markers. The risk score we observed indicated that average population risk scores are varied but on cumulating the ancestries the average risk score increased ~1.3 times than individual population average risk. The parameter which varies greatly while calculating the PRS is the ancestry; it should be prerequisite that individuals of same ancestry should be taken as a one population groups while calculating the scores.