The mining area in the Muli region, Qinghai Province, China, is an important source of water and an ecological security barrier in the Qilian Mountains region and has a very important ecological status. A series of ecological problems such as vegetation degradation and loss of biodiversity caused by mining have attracted widespread attention. In this paper, we used Landsat secondary data from 2000 to 2021 from the Muli region to obtain the spatial and temporal distribution characteristics of the vegetation in the Muli region by inversion of the fractional vegetation cover, above-ground biomass and the land surface phenology to comprehensively analyze the ecological changes in the vegetation in the Muli region. The results showed the following: (1) the above-ground biomass and cover of grassland in the Muli region showed a decreasing trend between 2000 and 2021, with a particularly pronounced decrease in grassland cover between 2009 and 2016; (2) the start of the vegetation growth cycle, i.e., the beginning of the vegetation growing season (SOG) became more advanced, the end of the vegetation growing season (EOG) was delayed, and the length of the growing cycle (LOG) became longer for most of the vegetation in the Muli region; (3) the results of this comprehensive analysis showed that the grassland in the Muli region showed dynamic changes with complex characteristics from 2000 to 2021, and anthropogenic disturbances had some influence on ecological indicators such as fractional vegetation cover and biomass. The extension of the vegetation growing season might be related to climate change. Based on the results of this paper, it is recommended to utilize biomass and fractional vegetation cover as indicators to assess the grass growth status of mining sites. This study analyzed the spatial and temporal characteristics of grasslands in the Muli area with several indicators, which will help relevant departments continue to improve and optimize ecological restoration measures. In addition, this study provides a reference for achieving comprehensive restoration of the ecological environment and ecological functions in mining areas.

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