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As a major food production crop in China, the growth and development of rice is an extremely complex systemic process, and the root system is the main organ for rice to obtain nutrients. Therefore, 3D modeling and visualization of the rice root system can help to further understand its morphology, structure and function, and provide an aid for scientific cultivation of rice and improving rice yield for decision making. In this paper, a mathematical model of the rice root system is established based on the B spline curve combined with the L-system approach, using mathematical knowledge based on the 3D morphological characteristics of the real rice root system. The B-Spline Curve is chosen to simulate this, and the recursive definition of B-Spline Curve and its formula are used to realize the modeling of the rice root system curve. Based on the mathematical method of rice root system integration, the bending effect of rice root system at different periods and different growth positions is realized. Finally, the L-system combined with B-Spline Curve is used to construct a rice root system model and realize the rice root system visualization simulation. The simulated image is closer to the real rice root system image in terms of morphological structure and has a strong sense of realism.