PURPOSE:To describe the vascular and tissue histopathological changes in seven sequential experimental liver transplantations in pigs. METHODS:Fourteen female pigs, Sus domesticus species, with body mass between 5 and 8 kg were utilized. After the end of all anastomoses of the graft implantation in the receptor, the animal was monitored for 30 minutes, and at its end one of the biopsies was collected for histological analysis. The histological criteria utilized were: lytic hepatocyte necrosis, density of septal and portal inflammatory infiltrated, sinusoidal congestion and hemorrhage. The analysis was performed separately for the portal region in zone 1, 2 and 3. RESULTS:Among the structural changes undergone by the graft, those with greater frequency and intensity were vascular congestion and steatosis, which stood out in transplantations 5, 6 and 7. CONCLUSIONS:The technique demonstrated vascular alterations represented by vasocongestion, edema and minimum inflammatory reaction. In relation to the parenchyma, was observed macrovacuolar pan-acinar steatosis, focal lytic and occasional hemorrhages, beyond the accumulation of hemosiderin in Kuppfer's cells.

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Introduction: taking into consideration the technical complexity involved in liver transplantation, the anesthetic-surgical team training, through experimental surgery on animals, has been considered a fundamental step before clinical application. Objective: to describe the technique modification of liver harvesting in orthotopic transplantation in pigs. Methods: seven sows Sus escrofa were used. The animals were previously anesthetized and placed in trans-operative monitoring; followed by the harvest of the organ by means of a different sequence than the reported by the literature, in which the liver vessels are handled in a clockwise direction. Results: the average time of hepatic harvesting was 84.4 minutes and the time of each anatomical structure dissection was considered satisfactory. Conclusion: the technique of liver harvesting in orthotopic transplantation in pigs described is viable, effective and reproducible, enabling the training of professionals and academics.

Objetivo: Descrever os procedimentos de hepatectomia total no receptor e de implante de enxerto hepático total em transplante ortotópico em suínos. Métodos: Foram utilizados 14 porcos Sus escrofa domesticus fêmeas, pesando entre 5 e 8 kg, os quais foram distribuídos em dois grupos: doadores e receptores, sendo considerados somente os últimos no resultado da presente pesquisa. Para iniciar o ato operatório, os animais foram previamente anestesiados e colocados em monitorização transoperatória, sendo em seguida submetidos aos procedimentos necessários para a hepatectomia do receptor e implante do enxerto total. Resultados: A cirurgia no animal receptor teve duração total média de 142,6 minutos, enquanto a fase anepática durou em média 64,6 minutos. Conclusão: As técnicas de hepatectomia em animal receptor e implante de enxerto total descritas são viáveis, eficazes e reprodutíveis, possibilitando o treinamento de profissionais e acadêmicos, bem como o desenvolvimento de futuras pesquisas envolvendo o transplante hepático.