Energy drinks are the beverage of choice for the 1 younger generation. A classical type of energy drink has a composition of water, sugar, caffeine in variable quantity, some bioactive compounds like guarana, glucoronolactone, and taurine, different types of 2 vitamin B like niacin, pantothenic acid, and cobalamin. Caffeine is a commonly consumed phytochemical, utilized both pharmacologically as well as industrially in beverage manufacturing. It is a centrally-acting stimulant affecting the nervous system by antagonizing 3 the adenosine receptors. It enhances the mental alertness and delays the feeling of fatigability when consumed in an energy drink, which possibly justifies its 4 role in these beverages. The glucose component in energy-enhancing beverages is usually responsible for providing a substrate required for physiological energy. They are also associated with the effects of the autonomic nervous system. There is strong evidence of a significant increase in both the pulse rate as well as

Objective: To evaluate the anatomical positions of vermiform appendix and its association with acute appendicitis on Multidetector computed tomography (MDCT). Study design and setting: A cross sectional study based on hospital database was carried out in Department of Anatomy in collaboration with Department of Radiology, Liaquat National Hospital. Methodology: A total of three hundred and six 306 adult urban patients CT axial images were evaluated retrospectively over period of 6 months from March 2021 to August 2021 who had abdominal MDCT scan for acute abdomen after getting approval from ethical committee to find out anatomical variations of position of vermiform appendix in a tertiary care hospital among adult urban Karachi population and its association with appendicitis. Data was analyzed using SPSS version 25. All categorical variables were summarized as frequencies and percentages. Result: In present study 159(52%) were males and 147(48%) were females with mean age of 32.97 years. The subcecal anatomical position of vermiform appendix on MDCT was most common n=85(27.8%), followed by postileal n=78(25.5%), pelvic n=61(19.9%), postcecal n= 36(11.8%), preileal n=29(9.5%), ectopic n=17(5.6%). The association between appendicitis with anatomical position of vermiform appendix and age was analyzed by chi- square and was statistically significant (p =0.05). There was no significant association between position of vermiform appendix on MDCT and gender. Conclusion: The subcecal position of vermiform appendix is most common on MDCT and the postileal position was more frequent in the inflamed group of vermiform appendix

Objectives: This study aimed to evaluate the anti-oxidative potential of ascorbic acid on methylphenidate induced decreased spermatogenesis and decreased testosterone levels in male Albino rats. Methods: This experimental study was performed on twelve-week-old Albino male rats selected through randomized sampling technique divided into three groups. Only healthy male rats of the same age and weighing between 180 to 200 gms were included, and any rat was appearing sick or had less weight than 180gms and aged less than 12 weeks were excluded. Control group A received 2 ml of normal saline for 50 days. Group B was given 2mg/day methylphenidate per oral for 50 days, and Group C received 2mg /day methylphenidate for 50 days, then ascorbic acid 50mg/day for 10 days. After 60 days, rats were dissected, testes were removed and histologically examined for spermatogenesis by Johnson's scoring, and blood samples were collected to evaluate testosterone levels. Results: On histological examination, the testes of animals of Group B showed a statistically significant decrease in Johnson's score (p-value < 0.001) with disruption of germinal epithelium and absence of mature sperms. These findings were associated with reduced levels of serum testosterone in this group. Administration of Ascorbic acid (AA) to group C showed statistically improved Johnson score and testosterone levels compared to group B. Conclusion: It has been concluded that administering Ascorbic Acid can improve decreased spermatogenesis and serum testosterone levels induced by methylphenidater

Objective: To observe the nephroprotective role of berberis vulgar is on renal parenchyma against microscopic and morphometric changes induced by Gentamicin in albino rats. Study Design: Lab based experimental study. Place and Duration of Study: It was conducted in Baqai Medical University in collaboration with department of Anatomy Liaquat National Hospital and Medical College, from Jan to Jul 2017. Methodology: A total of 40 male adult albino rats were used in the study. Four groups were made. Each group contained 10 rats. Group A was a control group, group B received only berberis vulgaris fruit extract orally per day for 21 days, group C received Gentamicin 100 mg/kg/day intraperitonially daily for 21 days. Group D received Gentamicin 100 mg/kg/day intraperitonially along with berberis vulgaris fruit extract 100 mg/kg/day orally. Both kidneys were removed. H&E and PAS stains were used for observing histological alterations and protective role of berberis vulgaris fruit extract. Results: Glomerulus and proximal convoluted tubules were observed histologically in all 4 groups. Microscopy of group B showed parameters nearly similar to control group. Microscopy of group C showed significant derangement in all parameters when compared with control group. Group C showed decrease glomerular, proximal convoluted tubular count was noted. Glomerular diameter increases and there was glomerular hypertrophy and tubular necrosis. Microscopy of group D showed significant improvement due to berberis vulgaris which restored normal renal architecture. Conclusion: Berberis vulgaris has a nephroprotective effect and it can be used as a new medicine against nephrotoxic drugs like Gentamicin.