The customer domain of the smart grid natu- rally blends with smart home and smart building systems, but typical proposed approaches are “distributor-centric” rather than “customer-centric,” undermining user acceptance, and are often poorly scalable. To solve this problem, we propose a detailed architecture and an implementation of a “last-meter” smart grid—the portion of the smart grid on customer premises—embedded in an internet-of-things (IoT) platform. Our approach has four aspects of novelty and advantages with respect to the state of the art: 1) seamless integration of smart grid with smart home applications in the same infrastructure; 2) data gathering from heterogeneous sensor communication protocols; 3) secure and customized data access; and 4) univocal sensor and actuator mapping to a common abstraction layer on which additional concurrent applications can be built. A demonstrator has been built and tested with purposely-developed ZigBee smart meters and gateways, a distributed IoT server, and a flexible user interface
Reducing energy consumption in datacenters is key to building low cost datacenters. To address this challenge, we explore the potential of hybrid datacenter designs that mix low power platforms with high performance ones. We show how these designs can handle diverse workloads with different service level agreements in an energy efficient fashion. We evaluate the feasibility of our approach through experiments and then discuss the design challenges and options of hybrid datacenters.
Agrobacterium tumefaciens infection of wounded plant tissues causes the formation of crown gall tumors. Upon infection, genes encoded on the A. tumefaciens tumor inducing plasmid are integrated in the plant genome to induce the biosynthesis of auxin and cytokinin, leading to uncontrolled cell division. Additional sequences present on the bacterial T-DNA encode for opine biosynthesis genes, which induce the production of opines that act as a unique carbon and nitrogen source for Agrobacterium. Crown galls therefore become a very strong sink for photosynthate. Here we found that the increased metabolic demand in crown galls causes an increase in oxygen consumption rate, which leads to a steep drop in the internal oxygen concentration. Consistent with this, plant hypoxia-responsive genes were found to be significantly upregulated in crown galls compared to uninfected stem tissue. Following this observation, we aimed at understanding whether the low-oxygen response pathway, mediated by group VII ethylene response factor (ERF-VII) transcription factors, plays a role in the development of crown galls. We found that quintuple knock-out mutants of all ERF-VII members, which are incapable of inducing the hypoxic response, show reduced crown gall symptoms. Conversely, mutant genotypes characterized by constitutively high levels of hypoxia-associated transcripts, displayed more severe crown gall symptoms. Based on these results, we concluded that uncontrolled cell proliferation of crown galls established hypoxic conditions, thereby requiring adequate anaerobic responses of the plant tissue to support tumor growth.
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