The article focuses on the design, optimization and implementation of a device for creating a laminar air curtain. Its purpose is to separate two spaces filled with air of different parameters, while these air masses do not mix. The function of the device is to prevent the transmission of respiratory pathogens that can be transmitted through the air. The premises can also be disinfected with a volatile inhalant. In the experimental device, air flow speeds are measured in two parts, namely in the part at the entrance of the air curtain to the chamber and in the part at the exit from it. Based on these measurements, velocity profiles are compiled, which are compared with verified simulations of the experimental device in CFD. The results indicate whether the device needs to be optimized, especially with regard to a more suitable geometry of the outlet and the laminar chamber. The new design is verified again in CFD simulation and finally implemented, so that it meets the requirements arising from the purpose of the device and is additionally verified by new measurements.