Modern squamates (lizards, snakes and amphisbaenians) are the world's most diverse group of tetrapods along with birds and have a long evolutionary history, with the oldest known fossils dating from the Middle Jurassic period-168 million years ago. The evolutionary origin of squamates is contentious because of several issues: (1) a fossil gap of approximately 70 million years exists between the oldest known fossils and their estimated origin; (2) limited sampling of squamates in reptile phylogenies; and (3) conflicts between morphological and molecular hypotheses regarding the origin of crown squamates. Here we shed light on these problems by using high-resolution microfocus X-ray computed tomography data from the articulated fossil reptile Megachirella wachtleri (Middle Triassic period, Italian Alps ). We also present a phylogenetic dataset, combining fossils and extant taxa, and morphological and molecular data. We analysed this dataset under different optimality criteria to assess diapsid reptile relationships and the origins of squamates. Our results re-shape the diapsid phylogeny and present evidence that M. wachtleri is the oldest known stem squamate. Megachirella is 75 million years older than the previously known oldest squamate fossils, partially filling the fossil gap in the origin of lizards, and indicates a more gradual acquisition of squamatan features in diapsid evolution than previously thought. For the first time, to our knowledge, morphological and molecular data are in agreement regarding early squamate evolution, with geckoes-and not iguanians-as the earliest crown clade squamates. Divergence time estimates using relaxed combined morphological and molecular clocks show that lepidosaurs and most other diapsids originated before the Permian/Triassic extinction event, indicating that the Triassic was a period of radiation, not origin, for several diapsid lineages.

[1] We performed a series of X-ray tomographic experiments and lattice Boltzmann permeability simulations on pyroclastic products from explosive activity at Stromboli between December 2004 and May 2006. We reconstructed the 3-D textures of vesicles to investigate the relationship between the nature of vesiculation in the erupted products and the dynamics of gas transport in the shallow conduit in order to derive implications for the eruptive behavior of basaltic volcanoes. Scoriae from normal Strombolian explosions display remarkably consistent vesicle volume distributions fit by power laws with an exponent of 1 (±0.2). We ascribe the origin of such distributions to the combined effect of coalescence and continuous nucleation events in the steady state, shallow magma system that supplies normal Strombolian activity. Volume distributions and textures of vesicles in pumice clasts from the 5 April 2003 and 15 March 2007 paroxysmal activity are markedly different from those in the scoriae. Besides a power law function with a higher exponent, portions of these distributions can be also fit by an exponential function, suggesting the attempt of the system to reach near-equilibrium conditions. The investigated pumice clasts also lack the large, connecting vesicles responsible for the development of degassing pathways in the Stromboli magma that erupts the scoriae. This testifies to a decreased degassing efficiency of the magma associated with paroxysmal explosions and potential overpressure buildup at depth. By comparison with degassing experiments on basaltic melts, we derive a time constraint on the order of minutes to hours for the incubation of paroxysms at Stromboli.

Synchrotron-radiation computed tomography has been applied in many research fields. Here, PITRE (Phase-sensitive X-ray Image processing and Tomography REconstruction) and PITRE_BM (PITRE Batch Manager) are presented. PITRE supports phase retrieval for propagation-based phase-contrast imaging/tomography (PPCI/PPCT), extracts apparent absorption, refractive and scattering information of diffraction enhanced imaging (DEI), and allows parallel-beam tomography reconstruction for conventional absorption CT data and for PPCT phase retrieved and DEI-CT extracted information. PITRE_BM is a batch processing manager for PITRE: it executes a series of tasks, created via PITRE, without manual intervention. Both PITRE and PITRE_BM are coded in Interactive Data Language (IDL), and have a user-friendly graphical user interface. They are freeware and can run on Microsoft Windows systems via IDL Virtual Machine, which can be downloaded for free and does not require a license. The data-processing principle and some examples of application will be presented.

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