It’s becoming even more widely recognized from neurosciences, epigenetics, and clinical research on observation of infant-caregiver interaction that daily cumulated micro-traumatic experiences cause damages not only to one’s mental health and identity, but also to immune system, leading to metabolic, eating, sleeping, affective, behavioural, cognitive and linguistic, and social disorders in adults as well as in children and infants. Relational Psychoanalytic treatment argues that the therapeutic change is related to expanding levels of consciousness and exploring new ways of being in the world. Clinical examples are provided.
TimMI è una équipe di professionisti esperti nel supporto delle fragilità familiari e nella prevenzione di ogni forma di maltrattamento all'infanzia. Nasce dalla collaborazione tra l'Ospedale dei Bambini Vittore Buzzi, dove ha sede, e Terre des Hommes. Questo articolo ne descrive la storia, gli obiettivi e le principali attività, presentando i dati delle osservazioni, acquisite tramite Intovian, dei casi seguiti e del servizio di supporto a minori e famiglie che hanno subito un ricovero per COVID-19.
specific share and predictive importance in the formation of impediment to the development of the fetus.The clinical retrospective study was carried out with occasion-control, traditional design. By random selection method, in the main group of 92 mothers were merged, whose pregnancy ended with the birth of a newborn with cervical development. The syndrome of fetal development of the fetus has been observed based on the non-conformity of the fetal data with the gestation period (after the 18th week of pregnancy with 10 percurrent indicators).The frequency and possible combinations of risk factors were analyzed during the material processing process. In the majority of cases of fetal development, the simultaneous existence of several factors of risk has been identified. Statistically significant risk factors have been defined as predictorative significance.The risk factors for high predictor significance have been identified: low levels of life (84%), endocrine pathology (82%), ischemic heart disease (85%), 85% of cases of cervical inflammation (85%), cervicitis (81%), chronic inflammation of ovaries (82%), endocrinologic pathology (82%), 86%) and the risk of pregnancy (80%). The predominant importance of preemaciation (92%) and minority (89%) was particularly high.In the study process, the mother's social-hygienic and medical-biological characteristics were studied. The risk factors, statistically significant, prioritized and high premedical significance have been identified, which can be detected at the preliminary stage. Timely identification and development of individual measures of management is important to minimize the exposure quality, to reduce the negative impact on pregnancy and the fetus.
The propagation of foreign DNA in Escherichia coli is central to molecular biology. Recent advances have dramatically expanded the ability to engineer (bacterial) cells; however, most of these techniques remain time-consuming. The aim of the present work was to explore the possibility to use the cloning-free genome editing (CFGE) approach, proposed by Döhlemann and coworkers (2016), for E. coli genetics, and to deepen the knowledge about the homologous recombination mechanism. The E. coli auxotrophic mutant strains FB182 (hisF892) and FB181 (hisI903) were transformed with the circularized wild-type E. coli (i) hisF gene and hisF gene fragments of decreasing length, and (ii) hisIE gene, respectively. His+ clones were selected based on their ability to grow in the absence of histidine, and their hisF/hisIE gene sequences were characterized. CFGE method allowed the recombination of wild-type his genes (or fragments of them) within the mutated chromosomal copy, with a different recombination frequency based on the fragment length, and the generation of clones with a variable number of in tandem his genes copies. Data obtained pave the way to further evolutionary studies concerning the homologous recombination mechanism and the fate of in tandem duplicated genes.
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