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A importância de um banco de dentes humanos em uma instituição de se justifica e se mostra vantajosa, uma vez que permite a máxima aproximação da realidade ao se trabalhar com o órgão extraído. Este estudo teve o propósito de avaliar métodos de manutenção da esterilidade do órgão dental humano extraído armazenado. Foram utilizados 72 dentes incisivos humanos extraídos, obtidos em clínicas de graduação e de pós-graduação da Universidade Positivo, Curitiba, PR, Brasil. Os elementos dentários foram cedidos pelos pacientes, por meio de termo de doação devidamente assinado. Após os procedimentos de limpeza e de desinfecção, 36 dentes foram esterilizados em autoclave e 36 foram somente limpos. Os dentes foram, então, armazenados em recipientes contendo o método (autoclavagem ou limpeza) ou a solução de escolha, por um período de 15 e 120 dias. Testes microbiológicos foram realizados a fim de determinar qual método ou solução de armazenamento promove a manutenção da esterilidade no tempo determinado. Melhores desempenhos foram observados quando as amostras foram armazenadas em Incidin Extra N®, formol e álcool, mesmo para os dentes não autoclavados. As substâncias em análise nos períodos propostos se mostraram capazes de impedir o crescimento microbiano. Este experimento poderá auxiliar o desenvolvimento de um protocolo de processamento e de administração do órgão dental humano extraído em um banco de dentes.

The biocompatibility of irrigant solutions, including 8.25% sodium hypochlorite (NaOCl), was analyzed in samples (n = 25) of chorioallantoic membranes (CAM) from embryos of the Gallus gallus, using the hen's egg test on chorioallantoic membrane (HET‐CAM). The following irrigants were tested: saline (control), 2% chlorhexidine (2% CHX), and NaOCl (2.5%, 5.25%, and 8.25%), and morphological and microcirculatory changes were investigated. Images were selected and quantified with Image J®. The macroscopic data were submitted to ANOVA, followed by the Tukey's test (p < .05); non‐parametric Kruskal‐Wallis and Mann–Whitney tests were applied to histological data (p < .05). The frequency of hyperemia differed significantly in the 8.25% NaOCl compared to the other groups, with the exception of 5.25% NaOCl (p = .096). Coagulation was more frequent in the 2% CHX group, differing significantly from the 2.5% NaOCl group (p = .038). In mean values, NaOCl 8.25% caused the decrease (NaOCl = 2.60, and control = 5.00) of vessels in the microcirculation, as well as triggered the increase (NaOCl = 425.50, and control 44.50) of inflammatory cells in the observed areas. There was necrosis in all samples in the 5.25% NaOCl group and in 80% in the 2% CHX, 2.5% NaOCl, and 8.25% NaOCl groups. Fibroblasts were detected only in the control group. It was concluded that the 8.25% NaOCl solution showed significantly high toxicity on CAM. However, these results were comparable to the other concentrations of NaOCl and to 2% CHX. Thus, in this experimental protocol, 8.25% NaOCl proved to be viable as an irrigant solution.

Introduction: Dental trauma affects mainly children, teenagers and young adults and the anterior teeth. In dental avulsion, the teeth are completely displaced out of the socket. This dentoalveolar lesion is that causing the most severe functional, esthetic, and psychologic damage to the patient. Objective: To evaluate the knowledge level of Dentistry undergraduates on tooth avulsion and verify whether the course period would be associated with the knowledge level. Material and methods: A questionnaire adapted from Fujita et al..[5], was applied, consisting of three parts, Part 1: general demographic data; Part 2: questions about the experience in tooth trauma Part 3: knowledge about tooth avulsion. The questionnaire was applied to 706 Dentistry undergraduates from Positivo University (PR), in June, 2015. The chi-square test was used to identify differences in responses for different variables, with significance level of 5%. Results: Most of the students were at the age range from 16 to 20 years-old (47.1%), were female (74.7%), were not trained on first-aid care in cases of tooth trauma (73.9%), and never faced a case of tooth avulsion (68.8%). The course period was statistically and positively associated with the knowledge on dental avulsion. Conclusion: The evaluated Dentistry undergraduate students knew how to manage cases of tooth avulsion. Statistically significant differences occurred in relation to the course period and the knowledge on tooth avulsion.

Root resorption, usually found through radiographic examinations is an internal or external injury. Since root resorption is a multidisciplinary problem, it requires a variety of knowledge for understanding the etiology and pathogenesis of the disease. Objective: This study aimed to demonstrate the importance of how a correct and early diagnosis facilitates the evaluation of the best treatment for each case, assuring a favorable prognosis. Case report: A radiographic finding in a 25-year-old young male showed the presence of an internal root resorption and apical periodontitis in tooth #22. After endodontic therapy and monthly calcium hydroxide dressing changes, the root canal filling was conducted and the case was radiographically followed-up. Conclusion: An accurate initial examination, with a carefully detailed anamnesis and periapical radiographs are vital points for a correct diagnosis, treatment and,