No abstract

The differentiation of germ cells in the adrenal glands of 26 male and female Swiss albino mice was studied in sequential stages of development, from day 12 1/2 of intrauterine life to postnatal day 21; the study was performed by means of high-resolution light microscopy and electron microscopy. In 12 1/2- and 13-day-old embryos, the ectopic cells had morphologic characteristics typical of primordial germ cells, whereas in 14- and 15-day-old fetuses they were identifiable as oogonia. In male and female fetuses from day 17 to term, all ectopic germinal elements entered meiotic prophase, reached diplotene, and differentiated into oocytes in perfect adherence to mouse ovarian timetables. In the postnatal animals, females as well as males, all oocytes progressed through the postmeiotic phase of growth just as they normally do in ovarian follicles, and, in the 2- and 3-week-old animals, they displayed features identical to those exhibited by oocytes in large antral follicles, including a zona pellucida. Germinal elements were no longer seen in the adrenals of animals older than 3 weeks. Our study shows that mammalian germ cells are capable of developing even outside the gonads, and that in ectopic sites they all differentiate as oocytes irrespective of their genetic sex.

No abstract

In the course of a study on the morphogenesis of the adrenal gland in random-bred Swiss albino mice, we noted the presence of ectopic germ cells in the adrenal cortexes and medullas in animals of both sexes, from day 12 1/2 of fetal development to postnatal day 12. Up to day 15 of fetal development, the cells exhibited characteristics of primordial germ cells. At day 17, and irrespective of the sex of the fetus, they all entered meiosis in synchrony with those in the ovary. Postnatally, in females as well as males, all ectopic germ cells displayed morphologic characteristics identical to those of young oocytes in unilaminar ovarian follicles. No germinal elements were seen in the adrenal glands past day 12 of life. Our study shows that mammalian germ cells are capable of undergoing sustained differentiation outside the gonads and that, in ectopic sites, they all differentiate into oocytes as they normally would in the ovary, even in males.