<p>La transferencia de plántulas de mora de condiciones <em>in vitro </em>a <em>ex vitro </em>es una de las fases más críticas de la técnica de micropropagación debido al alto grado de mortalidad de plántulas (50 a 90%), como consecuencia de una cutícula poco desarrollada, estomas no funcionales y un sistema radical débil que facilita la deshidratación por estrés hídrico. Esta investigación se orientó a la obtención de plántulas limpias procedentes de cultivo de tejidos y endurecidas con micorrizas arbusculares (HMA). La investigación se realizó bajo condiciones controladas; se utilizó un diseño experimental de bloques completos al azar con ocho tratamientos, tres repeticiones y cuatro unidades experimentales, así: tres tratamientos testigo sin inoculación, sin fertilizar (T0), con 50% de fertilización (T50), y con 100% de fertilización (T100), y cinco tratamientos inoculados con HMA (MA1, MA2, MA3, MA4 y Mycobiol) más T50. Los mayores beneficios de la inoculación con HMA se lograron con la cepa MA4 aislada de Silvania (Cundinamarca) y con esporas nativas clasificadas como <em>Glomus </em>sp. y <em>Acaulospora </em>sp. Las plantas inoculadas mostraron mejor adaptación al ambiente, expresada en el porte, la acumulación de biomasa foliar y radical, mayor área foliar y mejor estado nutricional expresado en una mayor absorción de nutrientes esenciales (P, N, Ca y Mg). El uso de la cepa MA4 permitió sustituir el 50% de la fertilización comercial debido a que obtuvo valores similares a T100 en la absorción de P y Ca, y superiores a ésta en la absorción de N y Mg. Este comportamiento vegetal se explicó por los niveles de colonización del hongo en las raíces.</p><p> </p><p><strong>Effect of arbuscular mycorrhizas on the acclimation and hardening of microplantlets of Andean blackberry (Rubus glaucus)</strong></p><p>The transfer of Andean blackberry plantlets from in vitro to ex vitro conditions is one of the most critical phases of the micropropagation technique due to the high mortality rate of plantlets (50-90%), as a consequence of a poorly developed cuticle, non-functional stomates and a weak radicle system that facilitates dehydration by water stress. This investigation focused on obtaining clean plantlets originated from tissue culture and hardened with arbuscular mycorrhizas (HMA). The research was performed under controlled conditions; an experimental design of random complete blocks was used with eight treatments, three repetitions and four experimental units as follows; three control treatments without inoculation, without fertilizing (T0), with 50% fertilizing (T50) and with 100% fertilizing (T100), and five treatments inoculated with HMA (MA1, MA2, MA3, MA4 and Mycobiol®) plus T50. The major benefits of inoculation with HMA were achieved with the strain MA4 isolated from Silvania (Cundinamarca) and with native spores classified as Glomus sp. and Acaulospora sp. The inoculated plants showed better adaptation to the environment, reflected in plant size, accumulation of foliar and radicle biomass, wider foliar area, and better nutritional state reflected in a higher absorption of essential nutrients (P, N, Ca, and Mg). The use of the strain MA4 allowed the substitution of 50% of commercial fertilization since it achieved similar values to T100 in the absorption of P and Ca, and higher absorption for N and Mg. The levels of root colonization by the fungus explained this vegetative behavior. </p>
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