Quantification of protein dimerization energies in lipid bilayers is hard to achieve, largely due to methodological challenges. Chadda et al. present an expansion of the single-molecule subunit-capture approach that incorporates empirical benchmarks for monomers and dimers, simplifying the process.

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9 10We previously reported the equilibrium dimerization reaction of the CLC-ec1 Cl -/H + transporter in 2:1 11POPE/POPG membranes (Chadda et al. 2016). This was determined by measuring the probability 21photobleaching data using this distribution shows that the protein is monomeric in the membrane and can 22serve as an experimental control. Dimer controls were constructed by glutaraldehyde cross-linking of 23C85A/H234C 'WT' or introducing R230C/L249C, which forms a spontaneous disulfide bond.

Ensilication is a novel method of protein thermal stabilisation using silica. It uses a modified sol–gel process which tailor fits a protective silica shell around the protein to enable room temperature storage of biopharmaceuticals.