Sensitive methods that are currently used to monitor proteolysis by plasmin in milk are limited due to their high cost and lack of standardisation for quality assurance in the various dairy laboratories. In this study, four methods, trinitrobenzene sulphonic acid (TNBS), reverse phase high pressure liquid chromatography (RP-HPLC), gel electrophoresis and fluorescamine, were selected to assess their suitability for the detection of proteolysis in milk by plasmin. Commercial UHT milk was incubated with plasmin at 37°C for one week. Clarification was achieved by isoelectric precipitation (pH 4.6 soluble extracts) or 6% (final concentration) trichloroacetic acid (TCA). The pH 4.6 and 6% TCA soluble extracts of milk showed high correlations (R2 > 0.93) by the TNBS, fluorescamine and RP-HPLC methods, confirming increased proteolysis during storage. For gel electrophoresis, extensive proteolysis was confirmed by the disappearance of α- and β-casein bands on the seventh day, which was more evident in the highest plasmin concentration. This was accompanied by the appearance of α- and β-casein proteolysis products with higher intensities than on previous days, implying that more products had been formed as a result of casein breakdown. The fluorescamine method had a lower detection limit compared with the other methods, whereas gel electrophoresis was the best qualitative method for monitoring β-casein proteolysis products. Although HPLC was the most sensitive, the TNBS method is recommended for use in routine laboratory analysis on the basis of its accuracy, reliability and simplicity.

Baobab (Adansonia digitata L.) is a majestic tree associated with human habitation in some of the semi-arid regions of Africa and establishes an enormous economic and nutritional importance to the rural residential districts. The fruit pulp is considered to be of high nutritional significance; particularly vitamin C and calcium, also possess antioxidant functions as well as high dietary fiber content. Although it is a potential fruit for improving local diets and livelihoods,this fruit is underutilized and its potential not yet fully acknowledged. This work was contracted with the aim of defining the nutritional quality and functional properties of baobab pulp harvested from some selected parts of Tanzania.Results indicated that the pulp from the three locations had moisture content which ranged between 9.16% to 10.30%, fat 0.46%-1.98%, ash 4.75%-5.21%, fiber 5.91%-9.65%, protein 3.23%-3.53%, carbohydrate 80.49%-85.19, vitamin C 169.74mg/100g-231.57mg/100g, beta-carotene 2.16 mg/100g-3.19mg/100g.Fructose 0.56±0.15-0.81±0.17g/100g, glucose 0.77±0.26-0.87±0.31g/100g and sucrose 0.75±0.25-0.84±0.29g/100g. The substantial differences (p≤ 0.05) between locations were observed in fat, crude fiber, carbohydrates, and fructose. Vitamin C, beta-carotene, protein, ash, moisture, sucrose and glucose showed no significance difference (p≤ 0.05) among locations. The functional properties included emulsification, foaming and gelling properties which ranged between 37.9-45.15%, 1.85-6.57% and 11-12% respectively and were significantly different (p≤ 0.05) among locations. The results show that baobab pulp has a good content of nutrients and functional properties which can be useful in food industries.

The fruits of Msasa and Jaffa orange varieties were harvested and subjected to post-harvest treatments namely; dipping in hexanal, dipping in calcium chloride and compared with untreated control; and they were stored at ambient (28 ± 2°C) and reduced temperatures (18 ± 2°C) conditions. Data were collected on the 0, 4 th , 8 th , and 12 th days from the date of fruit harvest. Chemical analyses were conducted to determine vitamin C content, total sugars, reducing sugars and total flavonoids. Post-harvest treatments had a significant effect on vitamin C and total flavonoids of Jaffa orange fruit. Hexanal treated fruit had higher values of total flavonoids, vitamin C, and total sugars compared to calcium chloride treated and control fruit. The storage durations showed significant effects on the vitamin C content of Jaffa, total flavonoids, the total and reducing sugars of both fruit varieties. Vitamin C and total flavonoids decreased with increasing storage duration of fruit. The significant interactions of factors were observed on total sugar which was increasing with increasing storage duration, but higher in hexanal treated and ambient stored fruit. Based on consumer acceptance test, the hexanal treated fruits were the most liked followed by calcium chloride treated and untreated fruits based on the appearance, taste, texture and overall acceptability.

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The effects of solar drying methods [Cabinet direct (CDD), cabinet mixed mode (CMD) and tunnel (TD) drying] on total phenolic content (TPC) and antioxidant capacities of commonly consumed fruits and vegetable in Tanzania were investigated. The TPC and FRAP in mangoes (Mangifera indica cv. Dodo, Viringe and Kent), bananas (Musa acuminata, cv. Kisukari, Kimalindi and Mtwike), pineapples (Ananas comosuss cv Smooth cayenne) and tomatoes (Lycopersicum esculentum cv. Tanya, Cal J and Onyx) were evaluated using Folin-Ciocalteu reagent and ferric reducing antioxidant power (FRAP) methods, respectively. There were significant (p<0.05) variations in TPC (mg GAE/100 g DM) and FRAP (µmol/100 g DM) among the fresh fruit and vegetable samples. The highest TPC was in tomato (476.6±8.6-538.9± 1.4) and lowest in banana (139.3±2.3-189.2±2.7). Drying methods had significant (p<0.05) effect on TPC and antioxidant values of the samples. All fresh samples had higher TPC levels but declined significantly in dried samples with the exception of tunnel dried tomatoes. Among the dried samples, the tunnel dried samples had less TPC loss (6-16%) than the cabinet dried samples (17-42%). The cabinet direct and mixed mode samples were not statistically different (p>0.05) from each other. However, tunnel dried samples had less FRAP loss (6-13%) which were statistically different (p<0.05) from the cabinet dried samples (14-56%). Percentage TPC and FRAP recoveries (%) differed significantly (P<0.05) between the varieties within the fruits/vegetable for both drying methods. A strong correlations between TPC and FRAP in both fresh (R 2 = 0.970) and dried samples (R 2 =0.8636) suggests that solar drying methods have significant effects on total phenolic contents and antioxidant activities of fruits and vegetables with tunnel drying method giving significantly less effects.