EDGE fixation induces complete PERV genetic material degradation in porcine aortic heart valves. This suggests that epoxy compounds may be alternatively used in the preparation of bioprosthetic heart valves in future.

The main problem with using modified allotrophic forms of carbon with nanodiamond particles in the production of food packaging is establishing the boundary between safety, as it affects the human body, and the adequate and effective action of the substances. One vital area of concern is the transmission of pathogens in food into the body. The aim of this study was to evaluate the cytotoxicity and bacteriostatic biological activity of two different modifications of diamond nanoparticles: pure detonation nanodiamond particles (DND) obtained by Danienko and plasma-chemically modified detonation nanodiamond particles obtained by the microwave plasma activated chemical vapor deposition method in a rotary chamber (MDP1) An indirect method was used to evaluate the cytotoxicity effect in accordance with ISO 10993–5. The viability of the L929 fibroblast cell line used as a control was 98.5%, for DND 95.14%, and the lowest level of viability for MDP1 was 88.63%. Escherichia coli and Staphylococcus aureus bacteria were used in bacteriostatic tests and the degree of cytotoxicity of the tested materials was classified as low. The in vitro cytotoxicity results indicate no toxic effect on L929 cells nor any effect on any of the samples tested against the bacterial strains us

A significant deficit in the availability of human tissues has not resulted in less general interest in homogeneous heart valves. The application of homografts still remains the optimal solution in the surgical treatment of congenital and acquired defects. Obtaining the optimal properties of these valves is followed by processing, including sterilization. This method provides sterility with rewarding cell viability and maintenance of the tissues’ native features. The prepared material possesses appropriate properties at the level of structure and surface, which results in correct functioning in vivo. During the past several decades, sterilization conditions and the compositions of antibiotic mixtures have changed significantly. In this period, significant progress in the development of this method was achieved; however, in the last few years, various flaws in antibiotic sterilization of homografts have been revealed. The release of the antibiotic during microbiological tests of tissue evaluated for implantation or the possibility of side effects as a result of antibiotic release after implantation remain the most important issues here. In this work we characterized the main antibiotic mixtures that are currently in use.

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