The paper outlines the experimental data on the impact of lyophilization on preservation of structural and functional characteristics of cells from human cord blood leukoconcentrate (HCBL). Lyophilization of HCBL was shown to ensure the preservation of cells with immune modulating potential in a heterogeneous population of HCBL. Lyophilized HCBL (lHCBL), to the same extent as cryopreserved one (cHCBL), revealed an equal immune correcting effect during ischemic brain injury in vivo in the experimental model of ischemic stroke (IS). The inclusion of lHCBL in therapy of brain ischemia demonstrated the improved indices of IFN-ү+ and IL-10+ spleen cells, adhesive and phagocytic activity of peritoneal cavity cells in rats with IS. The efficiency of using both lHCBL and cHCBL during IS may be associated with the implementation of therapeutic effect by presented among them cells and mediators, including multi-vector regulatory systems that maintain homeostatic stability of the body (immune, endocrine, nervous ones).

Cerebrovascular pathology, especially acute cerebrovascular accident is among the most complicated issues in medicine. Therefore, the search for efficient ways to treat neurodegenerative diseases has still remained topical. In experimental model of ischemic stroke (IS), the impact of lyophilized and cryopreserved human cord blood leukoconcentrate (lHCBL and cHCBL, respectively) on immune system humoral and cell components has been comparatively studied. The ischemic stroke was simulated in 6-monthold Wistar rats by occlusion of the middle cerebral artery. The lHCBL and cHCBL were administered intraperitoneally at a dose of 0.5 ml of 5 × 106 cells in 6 hrs after surgery (occlusion). In blood serum, the populations and subpopulations of lymphocytes (CD3+, CD4+, CD8+, CD16+, CD4+CD25+), the content of total immunoglobulins and circulating immune complexes were determined. The experimental model of IS showed both lyophilization and cryopreservation of HCBL to ensure the preservation of immunomodulatory potential of cells, that was confirmed in vivo. An equivalent corrective effect of lHCBL and cHCBL on indices of immune system cell and humoral components during IS development has been established. The inclusion of lHCBL, as well as cHCBL and native HCBL in basic therapy of IS promoted a more rapid recovery of the immune system indices in animals.