A rapid nonradioactive approach to the diagnosis of sickle cell anemia is described based on an allelespecific polymerase chain reaction (ASPCR). This method allows direct detection of the normal or the sickle cell g3-globin allele in genomic DNA without additional steps of probe hybridization, ligation, or restriction enzyme cleavage. Two allele-specific oligonucleotide primers, one specific for the sickle cell allele and one specific for the normal allele, together with another primer complementary to both alleles were used in the polymerase chain reaction with genomic DNA templates. The allele-specific primers differed from each other in their terminal 3' nucleotide. Under the proper annealing temperature and polymerase chain reaction conditions, these primers only directed amplification on their complementary allele. In a single blind study of DNA samples from 12 individuals, this method correctly and unambiguously allowed for the determination of the genotypes with no false negatives or positives. If ASPCR is able to discriminate all allelic variation (both transition and transversion mutations), this method has the potential to be a powerful approach for genetic disease diagnosis, carrier screening, HLA typing, human gene mapping, forensics, and paternity testing.Sickle cell anemia is the prototype ofa genetic disease caused by a single base-pair mutation, an A -* T transversion in the sequence encoding codon 6 of the human f3-globin gene. In homozygous sickle cell anemia, the substitution of a single amino acid (Glu -* Val) in the B-globin subunit of hemoglobin results in a reduced solubility of the deoxyhemoglobin molecule and erythrocytes assume irregular shapes. The sickled erythrocytes become trapped in the microcirculation and cause damage to multiple organs.Kan and Dozy (1) were the first to describe the diagnosis of sickle cell anemia in the DNA of affected individuals based on the linkage of the sickle cell allele to an Hpa I restriction fragment length polymorphism. Later, it was shown that the mutation itself affected the cleavage site of both Dde I and Mst II and could be detected directly by restriction enzyme cleavage (2, 3). Conner et al. (4) described a more general approach to the direct detection of single nucleotide variation by the use of allele-specific oligonucleotide hybridization. In this method, a short synthetic oligonucleotide probe specific for one allele only hybridizes to that allele and not to others under appropriate conditions. All of the above approaches are technically challenging, require a reasonably large amount of DNA, and are not very rapid. The polymerase chain reaction (PCR) developed by Saiki et al. (5) provided a method to rapidly amplify small amounts of a particular target DNA. The amplified DNA could then be readily analyzed for the presence of DNA sequence variation (e.g., the sickle cell mutation) by allelespecific oligonucleotide hybridization (6), restriction enzyme cleavage (5, 7), ligation of oligonucleotide pairs (8, 9), or ligation amplificatio...