Aerial images captured using infrared and green light showed noticeable differences. Apart from defocus, this did not affect centroid computations because within the variability of the techniques, estimates of aberrations with infrared were equivalent to those measured with green. In normal eyes, the Longitudinal Chromatic Aberration of the Indiana Chromatic Eye Model can predict the defocus term changes measured experimentally, although the intersubject variability could not be neglected. The largest deviations from the prediction were found on an aphakic eye and on the oldest subject.

We present an ocular adaptive optics system with a wavefront sampling rate of 240 Hz and maximum recorded closed-loop bandwidth close to 25 Hz, but with typical performances around 10 Hz. The measured bandwidth depended on the specific system configuration and the particular subject tested. An analysis of the system performance as a function of achieved bandwidth showed consistently higher Strehl ratios for higher closed-loop bandwidths. This may be attributed to a combination of limitations on the available technology and the dynamics of ocular aberrations. We observed dynamic behaviour with a maximum frequency content around 30 Hz.

Sequential measurement of ocular aberrations shows statistically significant differences in a large number of Zernike modes. If aberrations determined by a single measurement are to be used in a custom correction, the resulting modulation transfer function is likely to remain below the diffraction limit. Pupil realignment is critical in reduction of the residual root mean square wavefront values to a minimum.