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Pancreatic heterotopia is rare. Patients with pancreatic ectopia diagnosed by pathological study, whether asymptomatic or with mild symptoms, should be observed. Lesions incidentally detected during surgeries need to be removed by conservative procedures.

Two moderately halophilic Gram-negative bacteria were isolated from a sample taken from the brine-seawater interface of the Shaban Deep in the Red Sea. Phylogenetic analysis of the 16S rRNA gene sequence showed that these organisms represent a novel species of the genus Marinobacter. Cells of the new isolates formed non-pigmented colonies and were motile by means of a single polar flagellum. Strains SD-14BT and SD-14C grew optimally at 35-37 6C, in 5 % NaCl and at pH 7.5-8.0. The organisms were aerobic, but reduced nitrate to nitrogen under anaerobic conditions. Acid was produced from only a few carbohydrates. Ubiquinone 9 was the major respiratory quinone. The major fatty acids of strains SD-14B T and SD-14C were C 16 : 0 , C 18 : 1 v9c, summed feature 3 (C 16 : 1 v6c/C 16 : 1 v7c) and C 12 : 0 3-OH. The DNA G+C contents were 55.9 and 55.7 mol%, respectively. On the basis of the phylogenetic analyses and physiological and biochemical characteristics, it is proposed that strains SD-14B T and SD-14C represent a novel species of the genus Marinobacter, with the name Marinobacter salsuginis sp. nov. The type strain is strain SD-14B T (=DSM 18347 T =LMG 23697 T ).

The effect of altitude and season on abundance and diversity of the culturable heterotrophic bacterial and yeast community was examined at four forest sites in the Italian Alps along an altitude gradient (545–2000 m). Independently of altitude, bacteria isolated at 0 °C (psychrophiles) were less numerous than those recovered at 20 °C. In autumn, psychrophilic bacterial population increased with altitude. The 1194 bacterial strains were primarily affiliated with the classes Alpha-, Beta-, Gammaproteobacteria, Spingobacteriia and Flavobacteriia. Fifty-seven of 112 operational taxonomic units represented potential novel species. Strains isolated at 20 °C had a higher diversity and showed similarities in taxa composition and abundance, regardless of altitude or season, while strains isolated at 0 °C showed differences in community composition at lower and higher altitudes. In contrast to bacteria, yeast diversity was season-dependent: site- and altitude-specific effects on yeast diversity were only detected in spring. Isolation temperature affected the relative proportions of yeast genera. Isolations recovered 719 strains, belonging to the classes Dothideomycetes, Saccharomycetes, Tremellomycetes and Mycrobotryomycetes. The presence of few dominant bacterial OTUs and yeast species indicated a resilient microbial population that is not affected by season or altitude. Soil nutrient contents influenced significantly abundance and diversity of culturable bacteria, but not of culturable yeasts.Electronic supplementary materialThe online version of this article (doi:10.1007/s00792-016-0874-2) contains supplementary material, which is available to authorized users.

The microbial abundance and diversity at source, after bottling and through 6 months of storage of a commercial still natural mineral water were assessed by culture-dependent and culture-independent methods. The results revealed clear shifts of the dominant communities present in the three different stages. The borehole waters displayed low cell densities that increased 1.5-fold upon bottling and storage, reaching a maximum (6.2 × 10(8)  cells l(-1) ) within 15 days after bottling, but experienced a significant decrease in diversity. In all cases, communities were largely dominated by Bacteria. The culturable heterotrophic community was characterized by recovering 3626 isolates, which were primarily affiliated with the Alphaproteobacteria, Betaproteobacteria and Gammaproteobacteria. This study indicates that bottling and storage induce quantitative and qualitative changes in the microbial assemblages that seem to be similar as revealed by the two sample batches collected on 2 consecutive years. To our knowledge, this is the first study combining culture-independent with culture-dependent methods, and repeated tests to reveal the microbial dynamics occurring from source to stored bottled water.