Proteomics has had a big impact on plant biology, considered as a valuable tool for several forest species, such as Quercus, Pines, Poplars, and Eucalyptus. This review assesses the potential and limitations of the proteomics approaches and is focused on Quercus ilex as a model species and other forest tree species. Proteomics has been used with Q. ilex since 2003 with the main aim of examining natural variability, developmental processes, and responses to biotic and abiotic stresses as in other species of the genus Quercus or Pinus. As with the progress in techniques in proteomics in other plant species, the research in Q. ilex moved from 2-DE based strategy to the latest gel-free shotgun workflows. Experimental design, protein extraction, mass spectrometric analysis, confidence levels of qualitative and quantitative proteomics data, and their interpretation are a true challenge with relation to forest tree species due to their extreme orphan and recalcitrant (non-orthodox) nature. Implementing a systems biology approach, it is time to validate proteomics data using complementary techniques and integrate it with the -omics and classical approaches. The full potential of the protein field in plant research is quite far from being entirely exploited. However, despite the methodological limitations present in proteomics, there is no doubt that this discipline has contributed to deeper knowledge of plant biology and, currently, is increasingly employed for translational purposes.
PurposePipe‐spool module assembly involves a variety of uncertain factors and constraints, which complicate the assembly scheduling process. These factors also pose a challenge for the scheduler who struggles to produce a schedule that optimizes both the use of available resources (e.g. space) and meets the project's delivery deadlines. In the relatively quick production cycle of module assembly, the scheduling process plays an operative role. Thus, automation of the process would enhance productivity of the schedule updating process. This paper seeks to address this issue.Design/methodology/approachA simulation‐based approach is presented for scheduling pipe‐spool module assembly. This approach incorporates physical and logical constraints. General purpose simulation (GPS) is used to model these logical and heuristic constraints.FindingsThe application of the proposed model to an actual case study demonstrates the significant improvement in the assembly schedule when compared to traditional CPM‐based scheduling techniques.Originality/valueThe proposed model allows schedulers to experiment with various rules in order to improve the scheduling process by, for example, instantly updating the schedule.
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